Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, 13005 Marseille, France - IHU-Méditerranée Infection, 13005 Marseille, France.
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, 20705-2350 MD, United States.
Parasite. 2021;28:68. doi: 10.1051/parasite/2021066. Epub 2021 Oct 7.
Toxoplasma gondii oocysts are responsible for food- and water-borne infections in humans worldwide. They are resistant to common chemical disinfectants, including chlorinated products, presumably due to the structure and molecular nature of the oocyst wall but also the sporocyst wall. In this study, we used fluorescence microscopy and transmission electron microscopy to characterise the structure of both the oocyst and sporocyst walls, exposed to household bleach. Bleach removed the outer layer of the oocyst wall and the outer layer of the wall of sporocysts exposed due to rupture of the oocyst wall. The loss of the outer sporocyst wall layer was associated with a decrease in its autofluorescence, which can be linked to the degradation of dityrosine cross-link proteins, and loss of Maclura pomifera lectin-reactive glycoproteins. This study suggests that the inner layers of the oocyst and sporocyst walls are the main structures responsible for the resistance of the parasite to household bleach.
刚地弓形虫卵囊是造成全球人类食源性和水源性感染的原因。它们对常见的化学消毒剂具有抗性，包括含氯产品，这可能是由于卵囊壁和孢子囊壁的结构和分子特性，但也与它们有关。在这项研究中，我们使用荧光显微镜和透射电子显微镜来描述暴露于家用漂白剂时卵囊和孢子囊壁的结构。漂白剂去除了卵囊壁的外层和由于卵囊壁破裂而暴露的孢子囊壁的外层。孢子囊壁外层的丧失与它的自发荧光减少有关，这可以与二酪氨酸交联蛋白的降解以及 Maclura pomifera 凝集素反应性糖蛋白的丧失相关联。本研究表明，卵囊和孢子囊壁的内层是寄生虫对家用漂白剂产生抗性的主要结构。
