Bhushan Ashish, Kumari Ranbala, Srivastava Tapasya
Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021 India.
National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
3 Biotech. 2021 Oct;11(10):451. doi: 10.1007/s13205-021-02987-2. Epub 2021 Sep 26.
Investigating the therapeutic and prognostic potential of genes in the heterogeneous hypoxic niche of glioblastoma. We have analyzed RNA expression of U87MG cells cultured in hypoxia compared to normoxia. Common differentially expressed genes (DEGs) from GSE45301 and GSE18494 and their functional enrichment was performed using MetaScape and PANTHER. Hub genes and their ontology were identified using MCode cytoHubba and ClueGO and validated with GlioVis, Oncomine, HPA and PrognoScan. Using the GEO2R analysis of GSE45301 and GSE18494 datasets, we have found a total of 246 common DEGs (180 upregulated and 66 downregulated) and identified 2 significant modules involved in ribosome biogenesis and TNF signaling. Meta-analysis of key genes of each module in cytoHubba identified 17 hub genes (). Of the 17 hub genes, and were identified as hypoxia signatures associated with poor prognosis in Glioma. Ribosome biogenesis emerged as a vital contender of possible therapeutic potential with , , , and showing prognostic value.
The online version contains supplementary material available at 10.1007/s13205-021-02987-2.
研究胶质母细胞瘤异质性缺氧微环境中基因的治疗和预后潜力。我们分析了在缺氧条件下培养的U87MG细胞与正常氧条件下培养的细胞的RNA表达。使用MetaScape和PANTHER对来自GSE45301和GSE18494的常见差异表达基因（DEG）及其功能富集进行了分析。使用MCode cytoHubba和ClueGO鉴定了枢纽基因及其本体，并通过GlioVis、Oncomine、HPA和PrognoScan进行了验证。通过对GSE45301和GSE18494数据集的GEO2R分析，我们总共发现了246个常见的DEG（180个上调和66个下调），并确定了2个参与核糖体生物发生和TNF信号传导的重要模块。对cytoHubba中每个模块的关键基因进行荟萃分析，确定了17个枢纽基因（）。在这17个枢纽基因中，和被确定为与胶质瘤预后不良相关的缺氧特征。核糖体生物发生成为具有潜在治疗潜力的重要候选者，、、、和显示出预后价值。
在线版本包含可在10.1007/s13205-021-02987-2获取的补充材料。
