Department of Orthopedics, Zibo Central Hospital, Zibo Hospital Affiliated to Shandong First Medical University, Zibo, China.
Department of Clinical Laboratory, The Fifth People's Hospital of Zibo City, Zibo, China.
Bioengineered. 2021 Dec;12(2):9965-9975. doi: 10.1080/21655979.2021.1985343.
In recent researches, circular RNAs (circRNAs) have been shown to exert critical functions in osteosarcoma biology. Nevertheless, the contribution of circRNAs to osteosarcoma remains largely unclear. Results indicated that expression of circATRNL1 was higher in osteosarcoma tissues and cells. The high-expression of circATRNL1 was significantly correlated with aggressive features and acted as an independent risk factor for osteosarcoma patients' overall survival. Functionally, our findings demonstrate that circATRNL1 promotes the osteosarcoma aerobic glycolysis in vitro. Mechanistically, circATRNL1 up-regulated the expression level of LDHA, which was also targeted by miR-409-3p. Therefore, circATRNL1 exerted the accelerative roles of osteosarcoma aerobic glycolysis through miR-409-3p/LDHA axis. In conclusion, circATRNL1 promoted osteosarcoma progression by enhancing glycolysis via circATRNL1/miR-409-3p/LDHA axis, which may inspire a novel therapeutic target for osteosarcoma.
在最近的研究中，环状 RNA（circRNAs）被证明在骨肉瘤生物学中发挥着关键作用。然而，circRNAs 对骨肉瘤的贡献在很大程度上仍不清楚。结果表明，circATRNL1 在骨肉瘤组织和细胞中的表达水平较高。circATRNL1 的高表达与侵袭性特征显著相关，并作为骨肉瘤患者总生存的独立危险因素。功能上，我们的研究结果表明 circATRNL1 促进了骨肉瘤体外有氧糖酵解。在机制上，circATRNL1 上调了 LDHA 的表达水平，而 LDHA 也是 miR-409-3p 的靶点。因此，circATRNL1 通过 miR-409-3p/LDHA 轴发挥加速骨肉瘤有氧糖酵解的作用。总之，circATRNL1 通过 circATRNL1/miR-409-3p/LDHA 轴增强糖酵解促进骨肉瘤的进展，这可能为骨肉瘤提供新的治疗靶点。
