• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用抗原-抗体结合相互作用的 SARS-CoV-2 光学检测。

Optical Detection of SARS-CoV-2 Utilizing Antigen-Antibody Binding Interactions.

机构信息

Electrical Engineering Department, United Arab Emirates University, Al Ain 15551, United Arab Emirates.

Zayed Center for Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab Emirates.

出版信息

Sensors (Basel). 2021 Oct 2;21(19):6596. doi: 10.3390/s21196596.

DOI:10.3390/s21196596
PMID:34640920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8512587/
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease (COVID-19) pandemic, is sweeping the world today. This study investigates the optical detection of SARS-CoV-2, utilizing the antigen-antibody binding interactions utilizing a light source from a smart phone and a portable spectrophotometer. The proof-of-concept is shown by detecting soluble preparations of spike protein subunits from SARS-CoV-2, followed by detection of the actual binding potential of the SARS-CoV-2 proteins with their corresponding antigens. The measured binding interactions for RBD and NCP proteins with their corresponding antibodies under different conditions have been measured and analyzed. Based on these observations, a "hump or spike" in light intensity is observed when a specific molecular interaction takes place between two proteins. The optical responses could further be analyzed using the principle component analysis technique to enhance and allows precise detection of the specific target in a multi-protein mixture.

摘要

严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）是导致冠状病毒病（COVID-19）大流行的病毒，如今正在席卷全球。本研究利用智能手机光源和便携式分光光度计，利用抗原-抗体结合相互作用，对 SARS-CoV-2 进行光学检测。通过检测 SARS-CoV-2 的刺突蛋白亚单位的可溶性制剂，然后检测 SARS-CoV-2 蛋白与其相应抗原的实际结合潜力，证明了这一概念。测量了不同条件下 RBD 和 NCP 蛋白与其相应抗体的结合相互作用，并进行了分析。基于这些观察结果，当两种蛋白质之间发生特定的分子相互作用时，光强度会出现“驼峰或尖峰”。可以使用主成分分析技术进一步分析光学响应，以增强和允许在多蛋白混合物中精确检测特定目标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/d72de6b602f9/sensors-21-06596-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/e1e72d7f2ed7/sensors-21-06596-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/0f1cc8267bdb/sensors-21-06596-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/71b592a9a922/sensors-21-06596-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/6eba2ca0f31b/sensors-21-06596-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/903b43bc73a8/sensors-21-06596-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/80125c63cb1e/sensors-21-06596-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/7cce82374d58/sensors-21-06596-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/10b9be2c8040/sensors-21-06596-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/d72de6b602f9/sensors-21-06596-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/e1e72d7f2ed7/sensors-21-06596-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/0f1cc8267bdb/sensors-21-06596-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/71b592a9a922/sensors-21-06596-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/6eba2ca0f31b/sensors-21-06596-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/903b43bc73a8/sensors-21-06596-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/80125c63cb1e/sensors-21-06596-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/7cce82374d58/sensors-21-06596-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/10b9be2c8040/sensors-21-06596-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ed6/8512587/d72de6b602f9/sensors-21-06596-g009.jpg

相似文献

1
Optical Detection of SARS-CoV-2 Utilizing Antigen-Antibody Binding Interactions.利用抗原-抗体结合相互作用的 SARS-CoV-2 光学检测。
Sensors (Basel). 2021 Oct 2;21(19):6596. doi: 10.3390/s21196596.
2
Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity.针对 SARS-CoV-2 刺突受体结合域和核衣壳的异源抗体及其对 COVID-19 免疫的影响。
JCI Insight. 2020 Sep 17;5(18):142386. doi: 10.1172/jci.insight.142386.
3
Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays.采用微阵列和其他 12 种免疫分析法评估急性 SARS-CoV-2 感染中 S1、S2 和 NCP 特异性 IgM、IgA 和 IgG 抗体的动力学。
J Clin Microbiol. 2021 Apr 20;59(5). doi: 10.1128/JCM.02890-20.
4
Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana.在本氏烟中快速生产 SARS-CoV-2 受体结合域（RBD）和刺突蛋白特异性单克隆抗体 CR3022。
Sci Rep. 2020 Oct 19;10(1):17698. doi: 10.1038/s41598-020-74904-1.
5
Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.胶体金免疫层析法快速检测严重急性呼吸综合征冠状病毒 2 刺突蛋白的研究进展。
Front Immunol. 2021 Mar 11;12:635677. doi: 10.3389/fimmu.2021.635677. eCollection 2021.
6
Epitope Classification and RBD Binding Properties of Neutralizing Antibodies Against SARS-CoV-2 Variants of Concern.针对关注的 SARS-CoV-2 变体的中和抗体的表位分类和 RBD 结合特性。
Front Immunol. 2021 Jun 4;12:691715. doi: 10.3389/fimmu.2021.691715. eCollection 2021.
7
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter.使用基于HiBiT的生物报告基因检测严重急性呼吸综合征冠状病毒2受体结合域抗体
J Vis Exp. 2021 Aug 12(174). doi: 10.3791/62488.
8
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma.用于检测 COVID-19 恢复期血浆中 SARS-CoV-2 特异性 IgG 的多重冠状病毒抗原阵列的评估。
J Immunol Methods. 2021 Oct;497:113104. doi: 10.1016/j.jim.2021.113104. Epub 2021 Jul 22.
9
Quantitative SARS-CoV-2 Serology in Children With Multisystem Inflammatory Syndrome (MIS-C).儿童多系统炎症综合征（MIS-C）中 SARS-CoV-2 血清学的定量分析。
Pediatrics. 2020 Dec;146(6). doi: 10.1542/peds.2020-018242. Epub 2020 Sep 2.
10
Optimized Pseudotyping Conditions for the SARS-COV-2 Spike Glycoprotein.SARS-COV-2 刺突糖蛋白的优化假型条件。
J Virol. 2020 Oct 14;94(21). doi: 10.1128/JVI.01062-20.

引用本文的文献

1
Emerging Infectious Diseases Are Virulent Viruses-Are We Prepared? An Overview.新发传染病——烈性病毒来袭，我们准备好了吗？综述
Microorganisms. 2023 Oct 24;11(11):2618. doi: 10.3390/microorganisms11112618.
2
Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors.基于酶的电化学纳米生物传感器的新方法。
Biosensors (Basel). 2023 Jun 5;13(6):622. doi: 10.3390/bios13060622.
3
Special Issue "Computer Aided Diagnosis Sensors".特刊：计算机辅助诊断传感器

本文引用的文献

1
Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor.SARS-CoV-2 与 ACE2 受体结合的分子相互作用和抑制。
Nat Commun. 2020 Sep 11;11(1):4541. doi: 10.1038/s41467-020-18319-6.
2
Role of angiotensin-converting enzyme 2 (ACE2) in COVID-19.血管紧张素转化酶 2（ACE2）在 COVID-19 中的作用。
Crit Care. 2020 Jul 13;24(1):422. doi: 10.1186/s13054-020-03120-0.
3
Analysis of a SARS-CoV-2-Infected Individual Reveals Development of Potent Neutralizing Antibodies with Limited Somatic Mutation.对一名 SARS-CoV-2 感染者的分析显示，产生了具有有限体细胞突变的强效中和抗体。
Sensors (Basel). 2022 Oct 21;22(20):8052. doi: 10.3390/s22208052.
Immunity. 2020 Jul 14;53(1):98-105.e5. doi: 10.1016/j.immuni.2020.06.001. Epub 2020 Jun 8.
4
The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients.病毒刺突蛋白的受体结合域是 SARS-CoV-2 患者体内抗体的免疫优势和高度特异性靶标。
Sci Immunol. 2020 Jun 11;5(48). doi: 10.1126/sciimmunol.abc8413.
5
Enhanced receptor binding of SARS-CoV-2 through networks of hydrogen-bonding and hydrophobic interactions.通过氢键和疏水相互作用网络增强 SARS-CoV-2 的受体结合。
Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):13967-13974. doi: 10.1073/pnas.2008209117. Epub 2020 Jun 5.
6
Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen.开发一种便携式、超快速和超灵敏的基于细胞的生物传感器，用于直接检测 SARS-CoV-2 S1 刺突蛋白抗原。
Sensors (Basel). 2020 May 31;20(11):3121. doi: 10.3390/s20113121.
7
Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets.采用多重引物/探针组的 qRT-PCR 和 ddPCR 对 SARS-COV-2 的检测进行分析比较。
Emerg Microbes Infect. 2020 Dec;9(1):1175-1179. doi: 10.1080/22221751.2020.1772679.
8
SARS-CoV-2/COVID-19: Viral Genomics, Epidemiology, Vaccines, and Therapeutic Interventions.SARS-CoV-2/COVID-19：病毒基因组学、流行病学、疫苗和治疗干预措施。
Viruses. 2020 May 10;12(5):526. doi: 10.3390/v12050526.
9
Cell entry mechanisms of SARS-CoV-2.SARS-CoV-2 的细胞进入机制。
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734. doi: 10.1073/pnas.2003138117. Epub 2020 May 6.
10
Real-time RT-PCR in COVID-19 detection: issues affecting the results.实时逆转录聚合酶链反应在新冠病毒检测中的应用：影响结果的因素
Expert Rev Mol Diagn. 2020 May;20(5):453-454. doi: 10.1080/14737159.2020.1757437. Epub 2020 Apr 22.