Gholamalizadeh Maryam, Akbari Mohammad Esmail, Doaei Saeid, Davoodi Sayed Hossein, Bahar Bojlul, Tabesh Ghasem Azizi, Sadeghi Hossein, Razavi Hashemi Melika, Kheyrani Elham, Rastgoo Samira, Hajipour Azadeh, Aslany Zahra, Mirfakhraie Reza, Mosavi Jarrahi Alireza
Student Research Committee, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Oncol. 2021 Sep 28;11:732515. doi: 10.3389/fonc.2021.732515. eCollection 2021.
The association between the rs9939609 polymorphism of fat mass and obesity-associated gene (FTO) and risk of colorectal cancer is controversial. This study aims to evaluate the relationship between FTO rs9939609 polymorphism and colorectal cancer (CRC) in Iranian people.
A case-control study was conducted on 125 patients with CRC and 250 healthy subjects in Tehran, Iran. Demographic data and blood samples were collected from all participants. Genotyping of rs9939609 polymorphism was performed by the tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) method.
The occurrence of AA genotype of FTO rs9939609 polymorphism in the colorectal cancer patients was significantly higher compared to that of healthy subjects (16.4 2.9%, respectively, P=0.02). The association between the frequency of risk allele of the FTO polymorphism and CRC (B=1.67, P=0.042) remained significant after adjustment for age. Further adjustment for gender (model 2) and marital status (model 3) did not change this result (B=1.67, P= 0.042 and B=1.67, P=0.043, respectively). The results remained significant after additional adjustment for ethnicity (B=1.57, P= 0.047).
We found a positive association between the A allele of the rs9939609 polymorphism and CRC. Future studies are required to identify the underlying mechanisms.
脂肪量和肥胖相关基因（FTO）的rs9939609多态性与结直肠癌风险之间的关联存在争议。本研究旨在评估伊朗人群中FTO rs9939609多态性与结直肠癌（CRC）之间的关系。
在伊朗德黑兰对125例CRC患者和250名健康受试者进行了一项病例对照研究。收集了所有参与者的人口统计学数据和血液样本。采用四引物扩增阻滞突变系统-聚合酶链反应（T-ARMS-PCR）方法对rs9939609多态性进行基因分型。
与健康受试者相比，结直肠癌患者中FTO rs9939609多态性的AA基因型发生率显著更高（分别为16.4%和2.9%，P = 0.02）。在调整年龄后，FTO多态性风险等位基因频率与CRC之间的关联（B = 1.67，P = 0.042）仍然显著。进一步调整性别（模型2）和婚姻状况（模型3）并未改变这一结果（分别为B = 1.67，P = 0.042和B = 1.67，P = 0.043）。在额外调整种族后结果仍然显著（B = 1.57，P = 0.047）。
我们发现rs9939609多态性的A等位基因与CRC之间存在正相关。需要进一步的研究来确定其潜在机制。
