作为诱变剂的大肠杆菌K-12 dnaE突变体的分离。
Isolation of an Escherichia coli K-12 dnaE mutation as a mutator.
作者信息
Konrad E B
出版信息
J Bacteriol. 1978 Mar;133(3):1197-202. doi: 10.1128/jb.133.3.1197-1202.1978.
Abstract
Using a papillation method, a large number of Escherichia coli K-12 mutator mutations have been isolated. Only one of these (out of 1,250) mutator mutations has proved to be conditionally lethal at high temperatures. In vivo complementation tests indicated that this mutation, dnaE9, lies in dnaE, the structural gene for DNA polymerase III. The dnaE9 polymerase was not thermolabile in vitro; however, it showed a slow decline in specific activity in vivo at the nonpermissive temperature. Cultures of this mutant exhibited a comparably slow shutoff of DNA synthesis on shift to a nonpermissive temperature. dnaE9 showed temperature-sensitive mutator activity, which is not dependent on recA.
摘要
通过一种筛选方法，已分离出大量大肠杆菌K-12诱变突变体。这些突变体（共1250个）中只有一个在高温下被证明是条件致死的。体内互补试验表明，该突变体dnaE9位于DNA聚合酶III的结构基因dnaE中。dnaE9聚合酶在体外并非热不稳定；然而，在非允许温度下，其体内比活性显示出缓慢下降。该突变体培养物在转移到非允许温度时，DNA合成的关闭速度相对较慢。dnaE9表现出温度敏感的诱变活性，且不依赖于recA。
相似文献
1
Isolation of an Escherichia coli K-12 dnaE mutation as a mutator.作为诱变剂的大肠杆菌K-12 dnaE突变体的分离。
J Bacteriol. 1978 Mar;133(3):1197-202. doi: 10.1128/jb.133.3.1197-1202.1978.
2
Sequence analysis and phenotypes of five temperature sensitive mutator alleles of dnaE, encoding modified alpha-catalytic subunits of Escherichia coli DNA polymerase III holoenzyme.编码大肠杆菌DNA聚合酶III全酶修饰α催化亚基的dnaE五个温度敏感型突变等位基因的序列分析及表型
Mutat Res. 2002 Jan 29;499(1):85-95. doi: 10.1016/s0027-5107(01)00268-8.
3
Isolation of a dnaE mutation which enhances RecA-independent homologous recombination in the Escherichia coli chromosome.分离出一种增强大肠杆菌染色体中不依赖RecA的同源重组的dnaE突变。
Mol Microbiol. 1997 Jun;24(6):1225-34. doi: 10.1046/j.1365-2958.1997.4381795.x.
4
Characterization of arithmetic deoxyribonucleic acid synthesis at restrictive temperature in a dnaE mutant of Escherichia coli K-12.大肠杆菌K-12的dnaE突变体在限制温度下算术脱氧核糖核酸合成的特征分析。
J Bacteriol. 1979 Apr;138(1):201-6. doi: 10.1128/jb.138.1.201-206.1979.
5
Conditional lethality of Escherichia coli strains carrying dnaE and dnaQ mutations.携带dnaE和dnaQ突变的大肠杆菌菌株的条件致死性。
Mol Gen Genet. 1981;181(1):24-8. doi: 10.1007/BF00339000.
6
Genetic identification of two distinct DNA polymerases, DnaE and PolC, that are essential for chromosomal DNA replication in Staphylococcus aureus.金黄色葡萄球菌中对染色体DNA复制至关重要的两种不同DNA聚合酶DnaE和PolC的基因鉴定。
Mol Genet Genomics. 2001 Dec;266(4):564-71. doi: 10.1007/s004380100564. Epub 2001 Aug 16.
7
Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.DnaE蛋白（DNA聚合酶III的α亚基）的过量产生可恢复在DNA聚合酶I缺陷的条件性非存活大肠杆菌菌株中的生存能力。
J Bacteriol. 1992 Jun;174(12):4166-8. doi: 10.1128/jb.174.12.4166-4168.1992.
8
An Escherichia coli dnaE mutation with suppressor activity toward mutator mutD5.一种对诱变型mutD5具有抑制活性的大肠杆菌dnaE突变体。
J Bacteriol. 1992 Mar;174(6):1974-82. doi: 10.1128/jb.174.6.1974-1982.1992.
9
Isolation and characterization of a new globomycin-resistant dnaE mutant of Escherichia coli.大肠杆菌一种新的抗球霉素dnaE突变体的分离与鉴定
J Bacteriol. 1987 Aug;169(8):3400-8. doi: 10.1128/jb.169.8.3400-3408.1987.
10
Suppression of dnaE nonsense mutations by pcbA1.通过pcbA1抑制dnaE无义突变
J Bacteriol. 1989 Jun;171(6):3139-43. doi: 10.1128/jb.171.6.3139-3143.1989.
引用本文的文献
1
A Single Nucleotide Change in the DNA Polymerase III in Clostridium thermocellum Is Sufficient To Create a Hypermutator Phenotype.在产热梭菌的 DNA 聚合酶 III 中单个核苷酸的改变足以产生超突变表型。
Appl Environ Microbiol. 2022 Jan 11;88(1):e0153121. doi: 10.1128/AEM.01531-21. Epub 2021 Oct 20.
2
DNA polymerase switching: effects on spontaneous mutagenesis in Escherichia coli.DNA聚合酶转换：对大肠杆菌自发诱变的影响
Mol Microbiol. 2009 Jan;71(2):315-31. doi: 10.1111/j.1365-2958.2008.06526.x. Epub 2008 Nov 4.
3
Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.大肠杆菌RecQ DNA解旋酶在SOS信号传导及停滞复制叉处基因组稳定中的作用
Genes Dev. 2004 Aug 1;18(15):1886-97. doi: 10.1101/gad.1223804.
4
Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli.转座子Tn10衍生物精确切除频率增加的ssb和uup突变体的鉴定与特性分析：大肠杆菌中uup的核苷酸序列
J Bacteriol. 1997 May;179(9):2892-9. doi: 10.1128/jb.179.9.2892-2899.1997.
5
Mutants of Escherichia coli with increased fidelity of DNA replication.DNA复制保真度提高的大肠杆菌突变体。
Genetics. 1993 Aug;134(4):1023-30. doi: 10.1093/genetics/134.4.1023.
6
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
7
Identification of the epsilon-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication.鉴定大肠杆菌DNA聚合酶III全酶的ε亚基为dnaQ基因产物：DNA复制的保真亚基。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7085-9. doi: 10.1073/pnas.80.23.7085.
8
Isolation of conditional lethal mutator mutants of Escherichia coli by localized mutagenesis.通过局部诱变分离大肠杆菌的条件致死突变体突变株。
J Bacteriol. 1983 Mar;153(3):1361-7. doi: 10.1128/jb.153.3.1361-1367.1983.
9
New class of mutations in Escherichia coli (uup) that affect precise excision of insertion elements and bacteriophage Mu growth.大肠杆菌中影响插入元件精确切除和噬菌体 Mu 生长的一类新突变（uup） 。
J Bacteriol. 1983 Jan;153(1):384-9. doi: 10.1128/jb.153.1.384-389.1983.
10
Recombination genes on the Escherichia coli sex factor specific for transposable elements.大肠杆菌性因子上对转座元件特异的重组基因。
Proc Natl Acad Sci U S A. 1980 May;77(5):2814-8. doi: 10.1073/pnas.77.5.2814.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
3
Classification of aminotransferase (C gene) mutants in the histidine operon.组氨酸操纵子中氨基转移酶（C基因）突变体的分类
J Mol Biol. 1966 Nov 14;21(2):335-55. doi: 10.1016/0022-2836(66)90103-3.
4
Mapping of suppressor loci in Escherichia coli.大肠杆菌中抑制基因座的定位
J Mol Biol. 1965 Nov;14(1):153-66. doi: 10.1016/s0022-2836(65)80237-6.
5
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
6
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
7
Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.在与一类大肠杆菌K12 Rec-受体菌株交配时形成部分二倍体。
Proc Natl Acad Sci U S A. 1968 May;60(1):160-7. doi: 10.1073/pnas.60.1.160.
8
Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.脱氧核糖核酸复制缺陷的温度敏感型大肠杆菌突变体的分离与鉴定。
J Bacteriol. 1973 Mar;113(3):1381-8. doi: 10.1128/jb.113.3.1381-1388.1973.
9
Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.大肠杆菌K-12中条件性突变和营养缺陷型突变的快速定位
J Bacteriol. 1973 Feb;113(2):798-812. doi: 10.1128/jb.113.2.798-812.1973.
10
Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
Bacteriol Rev. 1972 Dec;36(4):587-607. doi: 10.1128/br.36.4.587-607.1972.
Zotero 插件