Zois Romanos, van Damme Mireille, Verbeek Martin, Veenendaal Luuk D H, Bai Yuling, Wolters Anne-Marie A
Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
Graduate School Experimental Plant Sciences, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
Theor Appl Genet. 2025 Sep 12;138(10):248. doi: 10.1007/s00122-025-05036-1.
The Tm-1 allele from Solanum pennellii accessions together with an additional, likely recessive, locus are required for complete ToBRFV resistance. The Tobamovirus Tomato Brown Rugose Fruit Virus (ToBRFV) poses a significant threat to global tomato production. ToBRFV is a mechanically transmitted virus containing a single-stranded positive sense RNA genome. Disease symptoms include brown, rough patches on fruit surfaces, leaf mosaicism and shape abnormalities, and, in advanced stages, total collapse of infected plants. ToBRFV was first detected in the Middle East in 2014 and has rapidly spread to multiple countries across Asia, Europe, and America. In recent years, numerous studies have focused on the identification of ToBRFV resistance traits that are suitable for tomato breeding programs. In this study, we identified five ToBRFV-resistant accessions of Solanum pennellii, a wild relative of cultivated tomato. We confirmed that the major gene controlling this resistance trait is the S. pennellii allele of Tm-1. Tm-1 was previously identified in S. habrochaites as a semidominant Tomato Mosaic Virus (ToMV) resistance gene. Our results show that full resistance to ToBRFV disease requires an additional undescribed locus. These results show the potential of S. pennellii as a novel source of resistance against ToBRFV.
来自潘那利番茄（Solanum pennellii）种质的Tm-1等位基因，连同另一个可能为隐性的位点，是番茄对番茄褐色皱纹果病毒（ToBRFV）产生完全抗性所必需的。番茄花叶病毒属病毒番茄褐色皱纹果病毒（ToBRFV）对全球番茄生产构成重大威胁。ToBRFV是一种机械传播的病毒，其基因组为单链正义RNA。病害症状包括果实表面出现褐色粗糙斑块、叶片花叶病和形状异常，在发病后期，受感染植株会完全枯萎。ToBRFV于2014年首次在中东地区被发现，并迅速传播到亚洲、欧洲和美洲的多个国家。近年来，众多研究聚焦于鉴定适合番茄育种计划的ToBRFV抗性性状。在本研究中，我们鉴定出了5份对ToBRFV具有抗性的潘那利番茄种质，潘那利番茄是栽培番茄的野生近缘种。我们证实，控制这种抗性性状的主要基因是Tm-1的潘那利番茄等位基因。Tm-1此前在多毛番茄（S. habrochaites）中被鉴定为一个半显性的番茄花叶病毒（ToMV）抗性基因。我们的结果表明，对ToBRFV病害的完全抗性还需要一个未描述的位点。这些结果显示了潘那利番茄作为ToBRFV新型抗性来源的潜力。
