Mei Haijun, Xian Hua, Ke Jing
Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
Front Mol Biosci. 2021 Sep 29;8:753218. doi: 10.3389/fmolb.2021.753218. eCollection 2021.
Infantile hemangioma (IH) is a common benign tumor of endothelial cells in infants. Most hemangiomas are self-limited, but a few may develop and lead to serious complications that affect the normal life of children. Therefore, finding an effective treatment strategy for IH is a pressing need. Recent studies have demonstrated that non-coding RNAs affect the progression of multiple tumors. This study aims to investigate the mechanism by which LncRNA-MCM3AP-AS1 promotes glycolysis in the pathogenesis of IH. We first documented that the expression of LncRNA MCM3AP-AS1 was significantly upregulated in IH. Furthermore, we demonstrated that MCM3AP-AS1 bound to miR-106b-3p which promotes glycolysis in IH. In addition, we found that inhibition of HIF-1α contributed to the transformation of glycolysis to normal aerobic oxidation, partially reversed the promoting effect on glycolysis by the up-regulation of LncRNA MCM3AP-AS1 in IH disease. More importantly, we demonstrated this phenomenon existed in IH patients. Taken together, we demonstrate that LncRNA-MCM3AP-AS1 promotes the progression of infantile hemangiomas by increasing the glycolysis via regulating miR-138-5p/HIF-1α axis.
婴儿血管瘤（IH）是婴儿常见的内皮细胞良性肿瘤。大多数血管瘤是自限性的，但少数可能发展并导致严重并发症，影响儿童的正常生活。因此，寻找一种有效的IH治疗策略迫在眉睫。最近的研究表明，非编码RNA影响多种肿瘤的进展。本研究旨在探讨长链非编码RNA-MCM3AP-AS1在IH发病机制中促进糖酵解的机制。我们首先记录到，长链非编码RNA MCM3AP-AS1在IH中的表达显著上调。此外，我们证明MCM3AP-AS1与促进IH中糖酵解的miR-106b-3p结合。此外，我们发现抑制缺氧诱导因子-1α（HIF-1α）有助于糖酵解转变为正常的有氧氧化，部分逆转了长链非编码RNA MCM3AP-AS1上调对IH疾病中糖酵解的促进作用。更重要的是，我们证明这种现象在IH患者中存在。综上所述，我们证明长链非编码RNA-MCM3AP-AS1通过调节miR-138-5p/HIF-1α轴增加糖酵解，从而促进婴儿血管瘤的进展。
