Srinivasan Sowmya, Jayasree R, Chennazhi K P, Nair S V, Jayakumar R
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682 041, India.
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682 041, India.
Carbohydr Polym. 2012 Jan 4;87(1):274-283. doi: 10.1016/j.carbpol.2011.07.058. Epub 2011 Aug 3.
Periodontal regeneration is of utmost importance in the field of dentistry which essentially reconstitutes and replaces the lost tooth supporting structures. For this purpose, nano bioactive glass ceramic particle (nBGC) incorporated alginate composite scaffold was fabricated and characterized using SEM, EDAX, AFM, FTIR, XRD and other methods. The swelling ability, in vitro degradation, biomineralization and cytocompatibility of the scaffold were also evaluated. The results indicated reduced swelling and degradation and enhanced biomineralization and protein adsorption. In addition, the human periodontal ligament fibroblast (hPDLF) and osteosarcoma (MG-63) cells were viable, adhered and proliferated well on the alginate/bioglass composite scaffolds in comparison to the control alginate scaffolds. The presence of nBGC enhanced the alkaline phosphatase (ALP) activity of the hPDLF cells cultured on the composite scaffolds. Thus results suggest that these biocompatible composite scaffolds can be useful for periodontal tissue regeneration.
牙周组织再生在牙科领域至关重要，其本质是重建和替换缺失的牙齿支持结构。为此，制备了掺入纳米生物活性玻璃陶瓷颗粒（nBGC）的海藻酸盐复合支架，并使用扫描电子显微镜（SEM）、能量散射X射线光谱仪（EDAX）、原子力显微镜（AFM）、傅里叶变换红外光谱仪（FTIR）、X射线衍射仪（XRD）等方法对其进行了表征。还评估了该支架的溶胀能力、体外降解、生物矿化和细胞相容性。结果表明其溶胀和降解减少，生物矿化和蛋白质吸附增强。此外，与对照海藻酸盐支架相比，人牙周膜成纤维细胞（hPDLF）和骨肉瘤（MG-63）细胞在海藻酸盐/生物玻璃复合支架上具有活力，能够良好地粘附和增殖。nBGC的存在增强了在复合支架上培养的hPDLF细胞的碱性磷酸酶（ALP）活性。因此，这些生物相容性复合支架可用于牙周组织再生。
