Zhang Jingfei, Han Hongli, Zhang Lili, Wang Tian
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Poult Sci. 2021 May;100(5):101061. doi: 10.1016/j.psj.2021.101061. Epub 2021 Feb 19.
This study was conducted to investigate the beneficial effects of bisdemethoxycurcumin (BDC) on growth performance, glutathione (GSH) redox potential, antioxidant enzyme defense, and gene expression in lipopolysaccharide (LPS)-challenged broilers. A total of 320, male, 1-day-old broilers were randomly assigned to 4 treatment groups including 8 replicates with 10 birds per cage in a 2 × 2 factorial arrangement: BDC supplementation (a basal diet with 0 or 150 mg/kg BDC) and LPS challenge (intraperitoneal injection of 1 mg/kg body weight saline or LPS at 16, 18, and 20 d of age). Results showed that dietary BDC supplementation prevented the LPS-induced decrease in ADG of broilers (P < 0.05). Compared to the saline-challenged group, LPS-challenged broilers showed higher jejunal and ileal malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) contents (P < 0.05). Dietary BDC supplementation alleviated LPS-induced increases in jejunal 8-OHdG, ileal MDA, and PC contents (P < 0.05). LPS challenge impaired the small intestinal antioxidant system, as evident by the decreases of GSH and total thiol contents, as well as superoxide dismutase (SOD), glutathione peroxidase, glutathione reductase (GR), and glutathione S-transferase (GST) activities. On the other hand, LPS challenge also increased GSH redox potential and oxidized glutathione (GSSG) contents (P < 0.05). Dietary BDC supplementation increased jejunal and ileal GSH contents, SOD activities, jejunal GR activity, and ileal GST activity, while it decreased jejunal and ileal redox potential, and jejunal GSSG contents (P < 0.05). Dietary BDC supplementation significantly alleviated the downregulation of mRNA expression levels of jejunal and ileal copper and zinc superoxide dismutase, catalytic subunit of γ-glutamylcysteine ligase, nuclear factor erythroid-2-related factor 2, heme oxygenase 1, NAD(P)H quinone oxidoreductase 1, and jejunal catalase and GR induced by LPS challenge (P < 0.05). In conclusion, BDC demonstrated favorable protection against LPS-induced small intestinal oxidative damages, as indicated by the improved growth performance, decreased GSH redox potential, enhanced antioxidant enzyme activities, and upregulated antioxidant-related gene expression.
本研究旨在探讨双去甲氧基姜黄素（BDC）对脂多糖（LPS）攻击的肉鸡生长性能、谷胱甘肽（GSH）氧化还原电位、抗氧化酶防御及基因表达的有益作用。总共320只1日龄雄性肉鸡被随机分为4个处理组，采用2×2析因设计，每笼10只鸡，每组8个重复：添加BDC（基础日粮中添加0或150mg/kg BDC）和LPS攻击（1日龄、18日龄和20日龄时腹腔注射1mg/kg体重的生理盐水或LPS）。结果表明日粮中添加BDC可防止LPS诱导的肉鸡平均日增重下降（P<0.05）。与生理盐水攻击组相比，LPS攻击的肉鸡空肠和回肠丙二醛（MDA）、蛋白质羰基（PC）和8-羟基-2'-脱氧鸟苷（8-OHdG）含量更高（P<0.05）。日粮中添加BDC可缓解LPS诱导的空肠8-OHdG、回肠MDA和PC含量增加（P<0.05）。LPS攻击损害了小肠抗氧化系统，表现为GSH和总硫醇含量以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶、谷胱甘肽还原酶（GR）和谷胱甘肽S-转移酶（GST）活性降低。另一方面，LPS攻击也增加了GSH氧化还原电位和氧化型谷胱甘肽（GSSG）含量（P<0.05）。日粮中添加BDC可增加空肠和回肠GSH含量、SOD活性、空肠GR活性和回肠GST活性，同时降低空肠和回肠氧化还原电位以及空肠GSSG含量（P<0.05）。日粮中添加BDC显著缓解了LPS攻击诱导的空肠和回肠铜锌超氧化物歧化酶、γ-谷氨酰半胱氨酸连接酶催化亚基、核因子红细胞2相关因子2、血红素加氧酶1、NAD（P）H醌氧化还原酶1以及空肠过氧化氢酶和GR mRNA表达水平的下调（P<0.05）。总之，BDC对LPS诱导的小肠氧化损伤具有良好的保护作用，表现为生长性能改善、GSH氧化还原电位降低、抗氧化酶活性增强以及抗氧化相关基因表达上调。
