State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Department of Animal Science, College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 250003, China.
Nutrients. 2021 Oct 6;13(10):3515. doi: 10.3390/nu13103515.
The metabolic disorder caused by excessive fructose intake was reported extensively and often accompanied by intestinal barrier dysfunction. And the rising dietary fructose was consumed at an early age of human. However, related researches were almost conducted in rodent models, while in the anatomy and physiology of gastrointestinal tract, pig is more similar to human beings than rodents. Hence, weaned piglets were chosen as the model animals in our study to investigate the fructose's impacts on intestinal tight junction, inflammation response and microbiota structure of piglets. Herein, growth performance, inflammatory response, oxidation resistance and ileal and colonic microbiota of piglet were detected after 35-day fructose supplementation. Our results showed decreased tight junction gene expressions in piglets after fructose addition, with no obvious changes in the growth performance, antioxidant resistance and inflammatory response. Moreover, fructose supplementation differently modified the microbiota structures in ileum and colon. In ileum, the proportions of Streptococcus and Faecalibacterium were higher in Fru group (fructose supplementation). In colon, the proportions of Blautia and Clostridium sensu stricto 1 were higher in Fru group. All the results suggested that tight junction dysfunction might be an earlier fructose-induced event than inflammatory response and oxidant stress and that altered microbes in ileum and colon might be the potential candidates to alleviate fructose-induced intestinal permeability alteration.
过量果糖摄入引起的代谢紊乱被广泛报道，常伴有肠道屏障功能障碍。而不断上升的饮食果糖摄入量在人类的幼年时期就已经开始了。然而，相关研究几乎都是在啮齿动物模型中进行的，而在胃肠道的解剖和生理学方面，猪比啮齿动物更接近人类。因此，我们选择断奶仔猪作为研究模型，以研究果糖对仔猪肠道紧密连接、炎症反应和微生物区系结构的影响。在此，在果糖补充 35 天后，检测了仔猪的生长性能、炎症反应、抗氧化能力以及回肠和结肠的微生物群。我们的结果表明，果糖添加后仔猪紧密连接基因表达降低，但生长性能、抗氧化能力和炎症反应无明显变化。此外，果糖补充还会改变回肠和结肠的微生物区系结构。在回肠中，Fru 组（果糖补充）中链球菌和粪杆菌的比例较高。在结肠中，Blautia 和严格梭菌 1 的比例在 Fru 组中较高。所有结果表明，紧密连接功能障碍可能是果糖诱导的炎症反应和氧化应激之前的事件，而回肠和结肠中改变的微生物可能是缓解果糖诱导的肠道通透性改变的潜在候选物。
