Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, Mexico.
Cátedras CONACYT-Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, Mexico.
Molecules. 2021 Oct 14;26(20):6195. doi: 10.3390/molecules26206195.
Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by HO, in brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM HO exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in brain cells by stimulating the activity of SOD and CAT.
水生生物体内的氧化应激可能会抑制免疫系统并传播传染病。本研究旨在通过体外模型，探究咖啡渣多酚提取物（SCG）对 HO 诱导的脑细胞氧化应激的保护作用。SCG 的亲水提取物富含奎宁酸、阿魏酸和咖啡酸，并且在 DPPH、ORAC 和 FRAP 测定中显示出抗氧化能力。此外，在 100µM HO 暴露（1 小时）前，将 SCG 多酚提取物（230 和 460µg/mL）预处理脑细胞 24 小时，可显著提高抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）并降低脂质过氧化（通过 MDA 水平衡量）。这些结果表明，SCG 提取物中的多酚通过刺激 SOD 和 CAT 的活性，对脑细胞氧化应激发挥抗氧化保护作用。
