Kwon Doo Yeon, Park Joon Yeong, Lee Bun Yeoul, Kim Moon Suk
Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea.
Polymers (Basel). 2020 Sep 26;12(10):2210. doi: 10.3390/polym12102210.
In this work, we prepared fluorescently labeled poly(ε-caprolactone-ran-lactic acid) (PCLA-F) as a biomaterial to fabricate three-dimensional (3D) scaffolds via salt leaching and 3D printing. The salt-leached PCLA-F scaffold was fabricated using NaCl and methylene chloride, and it had an irregular, interconnected 3D structure. The printed PCLA-F scaffold was fabricated using a fused deposition modeling printer, and it had a layered, orthogonally oriented 3D structure. The printed scaffold fabrication method was clearly more efficient than the salt leaching method in terms of productivity and repeatability. In the in vivo fluorescence imaging of mice and gel permeation chromatography of scaffolds removed from rats, the salt-leached PCLA scaffolds showed slightly faster degradation than the printed PCLA scaffolds. In the inflammation reaction, the printed PCLA scaffolds induced a slightly stronger inflammation reaction due to the slower biodegradation. Collectively, we can conclude that in vivo biodegradability and inflammation of scaffolds were affected by the scaffold fabrication method.
在本研究中，我们制备了荧光标记的聚（ε-己内酯-无规-乳酸）（PCLA-F）作为生物材料，通过盐析法和3D打印来制造三维（3D）支架。盐析法制备的PCLA-F支架是使用氯化钠和二氯甲烷制成的，具有不规则的、相互连接的三维结构。打印的PCLA-F支架是使用熔融沉积建模打印机制造的，具有分层的、正交取向的三维结构。就生产率和可重复性而言，打印支架的制造方法明显比盐析法更高效。在小鼠的体内荧光成像以及从大鼠体内取出的支架的凝胶渗透色谱分析中，盐析法制备的PCLA支架的降解速度比打印的PCLA支架略快。在炎症反应中，由于生物降解较慢，打印的PCLA支架引发的炎症反应略强。总体而言，我们可以得出结论，支架的体内生物降解性和炎症反应受支架制造方法的影响。
