Department of Translational Medicine, University of Ferrara, 44121 Ferrara, Italy.
Department of Chemistry, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, Italy.
Cells. 2021 Oct 8;10(10):2691. doi: 10.3390/cells10102691.
We investigated the phenolic content characterizing different plant extracts from , , and , their antioxidant, antiinflammatory effects, and their mechanism of action.
plant samples were macerated in 40% ethanol or hot/ cold glycerate and assessed for polyphenols content. The antioxidant activity was investigated by DPPH radical scavenging assay and HDCFDA test in LPS-stimulated RAW264.7 macrophages and N9 microglial cells. MTS experiments and antiinflammatory properties verified cellular toxicity through NO assay. Interaction with A adenosine receptors was evaluated through binding assays using [H]ZM241385 radioligand.
Polyphenols were present in 40% ethanol plant extract, which at 0.1-10 µg/µL achieved good antioxidant effects, with a DPPH radical scavenging rate of about 90%. In LPS-stimulated cells, these plant extracts, at 1μg/μL, did not affect cell vitality, displayed significant inhibition of HDCFDA and NO production, and inhibited ZM 241385 binding in CHO cells transfected with A receptors. RAW 264.7 and N9 cells presented a density of them quantified in 60 ± 9 and 45 ± 5 fmol/mg of protein, respectively.
, and extracts may be considered a source of agents for treating disorders related to oxidative stress and inflammation.
本研究旨在探讨不同植物提取物（ 、 、 ）的酚类成分特征、抗氧化、抗炎作用及其作用机制。
采用 40%乙醇或热/冷甘油提取法对植物样本进行浸提，检测多酚含量。采用 DPPH 自由基清除试验和 HDCFDA 试验评估 LPS 刺激 RAW264.7 巨噬细胞和 N9 小胶质细胞的抗氧化活性。通过 NO 测定验证 MTS 实验和抗炎特性的细胞毒性。采用 [H]ZM241385 放射性配体结合试验评估与 A 腺苷受体的相互作用。
40%乙醇植物提取物中存在多酚，在 0.1-10μg/μL 范围内可达到良好的抗氧化效果，DPPH 自由基清除率约为 90%。在 LPS 刺激的细胞中，这些植物提取物在 1μg/μL 时不影响细胞活力，显著抑制 HDCFDA 和 NO 的产生，并抑制 A 受体转染的 CHO 细胞中 ZM 241385 的结合。RAW 264.7 和 N9 细胞的密度分别为 60±9 和 45±5 fmol/mg 蛋白。
、 、 提取物可作为治疗与氧化应激和炎症相关疾病的药物来源。
