西班牙安达卢西亚西部采集的65种植物提取物的选择性抗癌活性筛选。

Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain.

作者信息

Calderón-Montaño José Manuel, Martínez-Sánchez Sara María, Jiménez-González Víctor, Burgos-Morón Estefanía, Guillén-Mancina Emilio, Jiménez-Alonso Julio José, Díaz-Ortega Patricia, García Felipe, Aparicio Abelardo, López-Lázaro Miguel

机构信息

Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.

Department of Vegetal Biology and Ecology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.

出版信息

Plants (Basel). 2021 Oct 15;10(10):2193. doi: 10.3390/plants10102193.

Finding cytotoxic drugs with a high selectivity towards cancer cells is crucial to improve the low survival rates of patients diagnosed with metastatic cancers. Since plants are an important source of anticancer drugs, we have screened 65 extracts from 45 plants collected in several areas of Western Andalusia (Spain) for cytotoxic activity on lung cancer cells versus lung normal cells. An extract from the leaves of (Vahl) Mast. (Cupressaceae) showed a marked cytotoxicity (IC50 = 0.37 ± 0.03 μg/mL) and selectivity (selectivity index = 378.3) against the lung cancer cells; cisplatin, 5-fluorouracil, and an extract from the leaves of L. (Taxaceae) were less cytotoxic and selective. Extracts from (L.) Lippold (Apocynaceae), Mill. (Rhamnaceae), subsp. (Pers.) Moreno (Brassicaceae), Sm (Cupressaceae), and L. (Amaryllidaceae) also showed selective cytotoxicity (selectivity index > 10). Active extracts were also tested against a panel of cancer cell lines from a variety tissues. The plants identified in this work are potential sources of natural compounds with selective toxicity towards cancer cells.

寻找对癌细胞具有高选择性的细胞毒性药物对于提高转移性癌症患者的低生存率至关重要。由于植物是抗癌药物的重要来源，我们筛选了从西班牙安达卢西亚西部几个地区收集的45种植物中的65种提取物，以检测其对肺癌细胞和肺正常细胞的细胞毒性活性。来自柏科植物刺柏（Juniperus oxycedrus (Vahl) Mast.）叶子的提取物对肺癌细胞显示出显著的细胞毒性（IC50 = 0.37 ± 0.03 μg/mL）和选择性（选择性指数 = 378.3）；顺铂、5-氟尿嘧啶以及来自红豆杉科植物欧洲红豆杉（Taxus baccata L.）叶子的提取物的细胞毒性和选择性较低。来自夹竹桃科植物罗布麻（Apocynum venetum (L.) Lippold）、鼠李科植物鼠李（Rhamnus cathartica Mill.）、十字花科植物芝麻菜亚种（Eruca sativa subsp. sativa (Pers.) Moreno）、柏科植物地中海柏木（Cupressus sempervirens Sm）和石蒜科植物水仙（Narcissus pseudonarcissus L.）的提取物也显示出选择性细胞毒性（选择性指数 > 10）。还针对一系列来自不同组织的癌细胞系对活性提取物进行了测试。这项工作中鉴定出的植物是对癌细胞具有选择性毒性的天然化合物的潜在来源。