Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, 228A College of Science and Technology Building No. 2 1-5-1 Kanda Surugadai Chiyoda, Tokyo, 101-0062, Japan.
Anal Sci. 2021 Nov 10;37(11):1491-1495. doi: 10.2116/analsci.21C009. Epub 2021 Oct 22.
Matrix-assisted laser desorption/ionization-based mass spectrometry imaging (MSI) of separated lipids on thin-layer chromatography (TLC) plates or followed by blotted hydrophilic polyvinylidene fluoride (PVDF) membranes has become a powerful tool in lipidomic analyses. However, background peaks in MS spectra often cover lipid peaks in a low amount/ionization effect; consequently, only low intensities/resolutions MSI are obtained. To address the aforementioned problem, we attempted 1,2-dichloroethane pre-washing of TLC plates before development and found that backgrounds could successfully be removed from the TLC plate or PVDF membrane.
基于基质辅助激光解吸/电离的质谱成像（MSI）技术可对薄层色谱（TLC）板上分离的脂质或印迹亲水聚偏二氟乙烯（PVDF）膜上的脂质进行分析，这一技术已经成为脂质组学分析的有力工具。然而，MS 光谱中的背景峰常常会覆盖低含量/电离效应的脂质峰；因此，只能得到低强度/分辨率的 MSI。为了解决上述问题，我们尝试在展开前用 1,2-二氯乙烷预清洗 TLC 板，发现可以成功地从 TLC 板或 PVDF 膜上去除背景。
