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携带合成大鼠α型转化生长因子基因的感染性逆转录病毒对正常大鼠肾（NRK）细胞的转化

Transformation of normal rat kidney (NRK) cells by an infectious retrovirus carrying a synthetic rat type alpha transforming growth factor gene.

作者信息

Watanabe S, Lazar E, Sporn M B

出版信息

Proc Natl Acad Sci U S A. 1987 Mar;84(5):1258-62. doi: 10.1073/pnas.84.5.1258.

DOI:10.1073/pnas.84.5.1258
PMID:3469667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304406/
Abstract

We synthesized a gene for rat type alpha transforming growth factor (TGF-alpha) consisting of the leader sequence and the sequence coding for the mature 50-amino acid peptide without the C-terminal processed region. This gene was inserted into the retrovirus vector pSW272, which is derived from spleen necrosis virus, to obtain an infectious recombinant virus carrying the rat TGF-alpha gene. This recombinant virus can infect normal rat kidney (NRK) cells and allow these cells to grow in soft agar in the presence of TGF-beta. Transformed cells isolated from colonies grown in soft agar contain an integrated form of the recombinant virus and secrete biologically active TGF-alpha into their medium. These results show that a biologically active TGF-alpha can be produced by a gene synthesized from only part of the coding region and that the infectious retrovirus carrying the TGF-alpha gene (SW355) can function as a transforming agent in NRK cells in the presence of TGF-beta.

摘要

我们合成了大鼠α型转化生长因子（TGF-α）基因，该基因由前导序列和编码成熟50个氨基酸肽的序列组成，不含C末端加工区域。将该基因插入源自脾坏死病毒的逆转录病毒载体pSW272中，以获得携带大鼠TGF-α基因的感染性重组病毒。这种重组病毒可以感染正常大鼠肾（NRK）细胞，并使这些细胞在TGF-β存在的情况下在软琼脂中生长。从在软琼脂中生长的菌落中分离出的转化细胞含有重组病毒的整合形式，并将生物活性TGF-α分泌到它们的培养基中。这些结果表明，仅由编码区域的一部分合成的基因可以产生生物活性TGF-α，并且携带TGF-α基因的感染性逆转录病毒（SW355）在TGF-β存在的情况下可以在NRK细胞中作为转化剂发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dce/304406/e9b2c3b1feed/pnas00270-0141-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dce/304406/5f059bd235c3/pnas00270-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dce/304406/e9b2c3b1feed/pnas00270-0141-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dce/304406/5f059bd235c3/pnas00270-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dce/304406/e9b2c3b1feed/pnas00270-0141-b.jpg

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