从 L. 中分离得到多糖，并对其进行结构解析，研究其抗氧化和降血糖活性。
Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of L.
机构信息
College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China.
Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
Molecules. 2022 May 7;27(9):3002. doi: 10.3390/molecules27093002.
The aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and it obtained a polysaccharide with yield of 21.802%. Then, a neutral polysaccharide named BRP-1-1 with a molecular weight of 31.378 kDa was isolated and purified from BRP using DEAE-650 M and Sephadex G-100. The structures of the BRP-1-1 were elucidated through a combination of FT-IR, GC-MS, NMR, and methylation analysis. The results showed that BRP-1 consisted of mannose (Man) and glucose (Glu) in a molar ratio of 7.62:1. The backbone of BRP-1-1 mainly consisted of →6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→, the branch was [T-α-D-Manp-(1]→. BRP-1-1 intervention significantly inhibited α-glucosidase activity; an inhibition rate of 44.623% was achieved at a concentration of 0.5 mg/mL. The results of the in vitro biological activity showed that BRP-1-1 has good antioxidant and hypoglycemic activity, suggesting that BRP-1-1 could be developed as a functional medicine.
本研究旨在探讨微波超声辅助提取（MUAE）对 L. 多糖（BRP）的含量、结构和生物功能的影响。采用响应面法（RSM）优化 MUAE 参数，得到得率为 21.802%的多糖。然后，从 BRP 中分离纯化出一种中性多糖 BRP-1-1，其分子量为 31.378 kDa。通过 FT-IR、GC-MS、NMR 和甲基化分析，对 BRP-1-1 的结构进行了阐明。结果表明，BRP-1 由甘露糖（Man）和葡萄糖（Glu）组成，摩尔比为 7.62:1。BRP-1-1 的糖链主要由→6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→组成，支链为[T-α-D-Manp-(1]→。BRP-1-1 干预显著抑制 α-葡萄糖苷酶活性；在 0.5 mg/mL 浓度下，抑制率达到 44.623%。体外生物活性结果表明，BRP-1-1 具有良好的抗氧化和降血糖活性，提示 BRP-1-1 可开发为功能性药物。
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