School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning, 530008, People's Republic of China.
School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning, 530008, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Sep 13;118(1):2. doi: 10.1007/s10482-024-02014-3.
A novel gram-stain-positive, short rod, aerobic, non-motile and non-spore-forming actinobacterial strain, designated GXG1230 was isolated from the rhizosphere soil of a coastal mangrove forest in Beihai city, Guangxi Zhuang Autonomous Region, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXG1230 was affiliated with the genus Microbacterium. Additionally, it demonstrated a high degree of similarity to Microbacterium paludicola US15 (97.9%) and Microbacterium marinilacus YM11-607 (97.3%). Chemotaxonomic characteristics showed that the whole-cell sugars were glucose, xylose, rhamnose and galactose. Menaquinones MK-11 and MK-12 were detected as respiratory quinones. Lysine was found in the peptidoglycan hydrolysate and the polar lipids were diphosphatidylglycerol, one phospholipid and two unidentified glycolipid. The major fatty acids were anteiso-C, iso-C and anteiso-C. The strain GXG1230 exhibited a genomic DNA G + C content of 71.7%. Furthermore, the average nucleotide identity values of GXG1230 with the reference strains were 75.4% and 81.9%, respectively, while the digital DNA-DNA hybridization values were 20.1% and 25.0%. Based on physiological, chemotaxonomic and phylogenetic information, strain GXG1230 is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium rhizophilus sp.nov is proposed, with GXG1230 (= MCCC 1K09302 = KCTC 59252) as the type strain.
一株新的革兰氏阳性、短杆状、需氧、非运动和非孢子形成的放线菌菌株，被命名为 GXG1230，是从中国广西壮族自治区北海市沿海红树林根际土壤中分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，菌株 GXG1230 与微杆菌属有关。此外，它与沼泽微杆菌 US15（97.9%）和海洋微杆菌 YM11-607（97.3%）具有高度相似性。化学分类特征表明，全细胞糖为葡萄糖、木糖、鼠李糖和半乳糖。检测到menaquinones MK-11 和 MK-12 作为呼吸醌。在肽聚糖水解物中发现赖氨酸，极性脂类为双磷脂酰甘油、一种磷脂和两种未鉴定的糖脂。主要脂肪酸为 anteiso-C、iso-C 和 anteiso-C。菌株 GXG1230 的基因组 DNA G+C 含量为 71.7%。此外，GXG1230 与参考菌株的平均核苷酸同一性值分别为 75.4%和 81.9%，而数字 DNA-DNA 杂交值分别为 20.1%和 25.0%。基于生理、化学分类和系统发育信息，菌株 GXG1230 被认为代表了微杆菌属的一个新种，提议将其命名为根际微杆菌新种，GXG1230（=MCCC 1K09302=KCTC 59252）为其模式菌株。
