Department of Genetics, Physiology and Microbiology (Unity of Animal Physiology), Faculty of Biology, Complutense University of Madrid (UCM), Madrid, Spain.
Institute of Investigation 12 de Octubre Hospital (i+12), Madrid, Spain.
Biogerontology. 2022 Feb;23(1):35-52. doi: 10.1007/s10522-021-09943-w. Epub 2021 Nov 2.
The ingestion of certain probiotics has been suggested as a promising nutritional strategy to improve aging. The objective of this work was to evaluate the effects of the daily intake, for a month, of a new probiotic Akkermansia muciniphila (AKK) (2 × 10 cfu/100µL PBS) on behavior, as well as function and redox state of immune cells of old female ICR-CD1 mice (OA group). For this, several behavioral tests were performed, and function and oxidative-inflammatory stress parameters of peritoneal leukocytes were analyzed in OA group, in a group of the same age that did not take AKK (old control, OC group) and in another adult control (AC) group. The results showed, in OA group, a significant improvement of several behavioral responses (coordination, balance, neuromuscular vigor, exploratory ability and anxiety like-behaviors), as well as in immune functions (chemotaxis, phagocytosis, NK activity and lymphoproliferation) and in oxidative stress parameters (glutathione peroxidase and reductase activities, oxidized glutathione and lipid oxidation concentrations) of the peritoneal leukocytes in comparison to those observed in OC group. In addition, peritoneal immune cells from the OA group released lower basal concentrations of pro-inflammatory cytokines (IL-2, IL-6 and TNF-α) compared to those from the OC group. The values of parameters in OA were similar to those in AC group. These improvements in the old mice receiving the probiotic were reflected in an increase in their lifespan. In conclusion, our data indicate that AKK supplementation for a short period could be a good nutritional strategy to promote healthy longevity.
某些益生菌的摄入被认为是一种有前途的营养策略，可以改善衰老。本工作的目的是评估每天摄入一种新的益生菌 Akkermansia muciniphila (AKK) (2×10cfu/100µL PBS) 一个月对老年雌性 ICR-CD1 小鼠 (OA 组) 行为以及免疫细胞功能和氧化还原状态的影响。为此，进行了多项行为测试，并分析了 OA 组、未服用 AKK 的同年龄组（老年对照组，OC 组）和另一个成年对照组（AC 组）的腹腔白细胞功能和氧化-炎症应激参数。结果表明，在 OA 组中，与 OC 组相比，几种行为反应（协调、平衡、神经肌肉活力、探索能力和焦虑样行为）、免疫功能（趋化性、吞噬作用、NK 活性和淋巴细胞增殖）以及氧化应激参数（谷胱甘肽过氧化物酶和还原酶活性、氧化型谷胱甘肽和脂质氧化浓度）均有显著改善。此外，与 OC 组相比，OA 组腹腔免疫细胞释放的基础促炎细胞因子（IL-2、IL-6 和 TNF-α）浓度较低。OA 组的参数值与 AC 组相似。在接受益生菌的老年小鼠中，这些改善反映在它们的寿命延长上。总之，我们的数据表明，短期补充 AKK 可能是促进健康长寿的一种良好营养策略。
