Oral Health Prev Dent. 2021 Nov 5;19:587-594. doi: 10.3290/j.ohpd.b2259087.
This in-situ-study investigated if rinsing the oral cavity with a calcium containing solution or a fluoride containing mouthwash immediately before an erosive attack leads to reduced enamel softening.
Bovine enamel samples (n = 240) with measured baseline surface microhardness (KHN) were assigned to five series (S1-5). Twelve participants carried out each series as follows: Four enamel samples of the associated test series were placed in an intraoral appliance and carried in each participants' mouth. After 30 min, the participants either rinsed the oral cavity for 60 s with 30 ml of a solution prepared from a 1,000 mg calcium effervescent tablet dissolved in 100 ml water (S2), an 800 mg calcium containing mineral supplement powder (5 g) dissolved in 200 ml water (S3), a fluoride (500 ppm) mouthwash (S4), a fluoride (500 ppm) and stannous chloride (800 ppm) containing mouthwash (S5), or did not rinse with any test solution before the erosive attack (S1, negative control). The participants subsequently rinsed the oral cavity with 100 ml of a soft drink (Sprite Zero) for 60 s to simulate the erosive attack and immediately afterwards with water to stop the erosive process. As final step, surface microhardness was measured a second time and hardness loss (∆KHN) calculated. Differences of ∆KHN between the series were investigated by fitting a mixed effect model to the data set.
The highest loss of microhardness and thus softening of enamel (mean of ∆KHN; lower/upper confidence level) was observed in the negative control (S1: 60.2; 67.6/52.8). While no statistically significant difference (P > 0.05) compared to S1 could be found in S2 (50.0; 57.4/42.5) and S3 (54.6; 62.1/47.2), statistically significantly less softening of enamel (P < 0.001) was discovered in S5 (33.8; 41.2/26.4) and S4 (41.8.2; 49.3/34.4). S5 showed the overall lowest values for ∆KHN and thus best protection from enamel softening.
Rinsing with a fluoride mouthwash or a fluoride and stannous chloride containing mouthwash immediately before an erosive attack reduces the softening of enamel. None of the investigated calcium-containing solutions was able to reduce erosion induced softening of enamel.
本原位研究旨在探讨在侵蚀攻击前立即用含钙溶液或含氟漱口水漱口是否会减少牙釉质软化。
用测量的基线表面显微硬度（KHN）对 240 个牛牙釉质样本进行分组（S1-5）。十二名参与者按以下方式进行每项系列研究：将相关测试系列的四个牙釉质样本放置在口腔内装置中，并由每位参与者携带。30 分钟后，参与者用 30 毫升溶液漱口 60 秒，溶液由一片 1000mg 泡腾钙片溶解在 100ml 水中制成（S2），由 800mg 含钙矿物质补充剂粉末（5g）溶解在 200ml 水中制成（S3），由含氟（500ppm）漱口水（S4），由含氟（500ppm）和氯化亚锡（800ppm）漱口水（S5）漱口，或在侵蚀攻击前不使用任何测试溶液漱口（S1，阴性对照）。参与者随后用 100ml 雪碧（Sprite Zero）漱口 60 秒以模拟侵蚀攻击，然后立即用清水停止侵蚀过程。作为最后一步，再次测量表面显微硬度并计算硬度损失（∆KHN）。通过将混合效应模型拟合到数据集来研究各系列之间的 ∆KHN 差异。
在阴性对照组（S1：60.2；67.6/52.8）中观察到牙釉质硬度损失最大（∆KHN 均值；置信下限/上限），因此牙釉质软化程度最高。与 S1 相比，S2（50.0；57.4/42.5）和 S3（54.6；62.1/47.2）中没有发现统计学上的显著差异（P>0.05），但在 S5（33.8；41.2/26.4）和 S4（41.8；49.3/34.4）中发现了统计学上显著的牙釉质软化程度较低（P<0.001）。S5 显示出∆KHN 的总体最低值，因此对牙釉质软化的保护效果最佳。
在侵蚀攻击前立即用含氟漱口水或含氟和氯化亚锡漱口水漱口可减少牙釉质的软化。所研究的含钙溶液均不能减少由侵蚀引起的牙釉质软化。
