Laboratory of Applied Sports Science, Institute of Physical Education and Sports, Federal University of Alagoas, Avenue Lourival Melo Mota, s/n, Maceió, AL, 57072-900, Brazil.
Postgraduate Nutrition Program - PPGNUT, Faculty of Nutrition, Federal University of Alagoas, Maceió, Brazil.
Eur J Appl Physiol. 2022 Feb;122(2):371-382. doi: 10.1007/s00421-021-04838-7. Epub 2021 Nov 5.
The study aimed to assess the metabolic impact of elite Brazilian U-20 players using the rating of perceived exertion scale (RPE) to discriminate metabolomics sensitivity post-two soccer games separated by a short recovery interval.
Urine was collected immediately and then 20 h after two soccer matches of elite Brazilian U-20 players. RPE was collected after games. The spectra were pre-processed using TopSpin3.2 software. Chenomxsoftware was used to identify metabolites in the urine through the available database.
The results showed that the metabolic pathways related to energy production, cellular damage, and organic stresses were changed immediately after the game. 20 h after the games, antioxidant and anti-inflammatory pathways related to cell recovery were identified (e.g., gallic acid, ascorbate, and betaine). The matrix of positive correlations between metabolites was more predominant and stronger after game 2 than game 1. T-distribution registered metabolites discriminated below and above 7 on the RPE scale. Athletes with higher RPE values showed a high metabolite profile related to muscle damage (e.g., creatine, creatinine, and glycine) and energy production (e.g., creatine, formate, pyruvate, 1,3 dihydroxyacetone) 20 h post-soccer match. There was a different metabolic profile between athletes with higher and lower RPE values.
Metabolomics analysis made it possible to observe the metabolic impacts of energy production and muscular damage. RPE identified internal load changes within the group as a result of match intensity in soccer. The correlation matrix indicated a greater predominance of positive and strong correlations between metabolites in the second game compared to the first game.
本研究旨在评估精英巴西 U-20 球员的代谢影响，使用感知用力评分（RPE）在两次足球比赛之间进行短暂恢复后区分代谢组学的敏感性。
在两次精英巴西 U-20 球员的足球比赛后立即和 20 小时收集尿液。比赛后收集 RPE。使用 TopSpin3.2 软件对光谱进行预处理。Chenomxsoftware 用于通过可用数据库识别尿液中的代谢物。
结果表明，比赛后即刻的能量产生、细胞损伤和有机应激相关代谢途径发生变化。20 小时后，确定了与细胞恢复相关的抗氧化和抗炎途径（例如，没食子酸、抗坏血酸和甜菜碱）。与代谢物相关的正相关矩阵在第二次比赛后比第一次比赛更主要和更强。T 分布登记的代谢物在 RPE 量表上低于和高于 7 时可区分。RPE 值较高的运动员表现出与肌肉损伤（例如肌酸、肌酐和甘氨酸）和能量产生（例如肌酸、甲酸盐、丙酮酸、1,3 二羟基丙酮）相关的高代谢物谱 20 小时后足球比赛。RPE 值较高和较低的运动员之间存在不同的代谢谱。
代谢组学分析使观察能量产生和肌肉损伤的代谢影响成为可能。RPE 根据足球比赛的强度识别组内的内部负荷变化。相关矩阵表明，与第一次比赛相比，第二次比赛中代谢物之间的正相关和强相关更为主要。
