Park Soongho, Nguyen Thien, Benoit Emilie, Sackett Dan L, Garmendia-Cedillos Marcial, Pursley Randall, Boccara Claude, Gandjbakhche Amir
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Dr., Bethesda 20814, USA.
LLTech SAS-Aquyre Biosciences, 58 Rue du Dessous des Berges, 75013 Paris, France.
Biomed Opt Express. 2021 Sep 21;12(10):6431-6441. doi: 10.1364/BOE.436330. eCollection 2021 Oct 1.
Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.
动态全场光学相干显微镜（DFFOCM）用于表征处于不同活力状态的HeLa细胞的细胞内动态活动和细胞骨架。对HeLa细胞样本进行了24小时的连续监测，并与组织学检查进行比较以确认细胞活力状态。在健康细胞中观察到的平均频率和幅度分别为4.79±0.5 Hz和2.44±1.06。在死亡细胞中，平均频率移至8.57±0.71 Hz，而幅度显著降至0.53±0.25。预计这种使用DFFOCM的细胞动态活动分析将取代传统的耗时且需要活检的组织学或生化方法。
