Apelian Clement, Harms Fabrice, Thouvenin Olivier, Boccara A Claude
Institut Langevin-ESPCI ParisTech, PSL Research University 1 Rue Jussieu, 75005, Paris, France; LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
LLTech SAS, Pépinière Paris Santé Cochin 29 Rue du Faubourg Saint Jacques, 75014, Paris, France.
Biomed Opt Express. 2016 Mar 24;7(4):1511-24. doi: 10.1364/BOE.7.001511. eCollection 2016 Apr 1.
We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.
我们开发了一种新的内源性方法，利用全场光学相干断层扫描干涉信号的时间依赖性，揭示新鲜离体组织中的亚细胞代谢对比度。该方法揭示了与内源性散射元件局部活性相关的信号，这些信号可以揭示其他基于光学相干断层扫描的技术无法检测到或需要外源性造影剂的细胞。我们受益于全场光学相干断层扫描的微米级横向分辨率来成像细胞内特征。我们利用这种时间依赖性，在正常或病理条件下，在广泛的器官上识别毫秒级的不同动力学。
