Soheilikhah Zhaleh, Modarresi Masoud, Karimi Naser, Movafeghi Ali
Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Heliyon. 2021 Oct 20;7(10):e08228. doi: 10.1016/j.heliyon.2021.e08228. eCollection 2021 Oct.
Hyssop ( L.) is a perennial subshrub, which is distributed across the eastern Mediterranean region to central Asia. One of the most important bioactive compounds of hyssop is diosmin, a flavone glycoside of diosmetin, with application in the field of cardiovascular therapy. Salinity as one of the most essential environmental stress factors is able to alter secondary metabolite content in plants. Therefore, we aimed to investigate the effect of salinity on the levels of total flavonoid content and diosmin in hyssop. Accordingly, salinity stress was imposed by watering plants with four different concentrations of sodium chloride (NaCl) (50, 100, 150 and 200 mM) for 4 weeks. High-performance liquid chromatography (HPLC) method was used for purification of diosmin from dried leaves and measurement of it in dried shoots. Nuclear magnetic resonance (NMR) spectroscopy was applied for determination of the structure of diosmin. The obtained results showed that high salinity levels lead to a higher amount of total flavonoid and diosmin content in treated plants. Although alteration in diosmin content was not significant in treatments up to 100 mM NaCl, higher amounts of diosmin were observed in 150 and 200 mM NaCl salinity levels. We concluded that the contents of total flavonoid and diosmin were significantly elevated after exposure of hyssop plants to salt stress conditions.
神香草（L.）是一种多年生亚灌木，分布于地中海东部地区至中亚。神香草最重要的生物活性化合物之一是香叶木苷，它是香叶木素的黄酮糖苷，应用于心血管治疗领域。盐度作为最重要的环境胁迫因素之一，能够改变植物中次生代谢产物的含量。因此，我们旨在研究盐度对神香草中总黄酮含量和香叶木苷水平的影响。据此，通过用四种不同浓度的氯化钠（NaCl）（50、100、150和200 mM）浇灌植物4周来施加盐胁迫。采用高效液相色谱（HPLC）法从干燥叶片中纯化香叶木苷并在干燥茎中进行测定。应用核磁共振（NMR）光谱法测定香叶木苷的结构。所得结果表明，高盐度水平导致处理过的植物中总黄酮和香叶木苷含量更高。尽管在高达100 mM NaCl的处理中香叶木苷含量的变化不显著，但在150和200 mM NaCl盐度水平下观察到了更高含量的香叶木苷。我们得出结论，神香草植物在盐胁迫条件下暴露后，总黄酮和香叶木苷的含量显著升高。
