Department of Biology, Faculty of Science, Al-Baha University, Al-Baha, 65799 Saudi Arabia.
Chemistry Department, College of Science, Jouf University, Sakaka, Saudi Arabia.
Int J Nanomedicine. 2024 Feb 14;19:1469-1485. doi: 10.2147/IJN.S443965. eCollection 2024.
Nowadays, recycling agricultural waste is of the utmost importance in the world for the production of valuable bioactive compounds and environmental protection. Olive leaf bioactive compounds have a significant potential impact on the pharmaceutical industry. These compounds possess remarkable biological characteristics, including antimicrobial, antiviral, anti-inflammatory, hypoglycemic, and antioxidant properties.
The present study demonstrates a green synthetic approach for the fabrication of nickel oxide nanoparticles (NiO-olive) using aqueous wasted olive leaf extract. Calcination of NiO-olive at 500°C led to the fabrication of pure NiO nanoparticles (NiO-pure). Different techniques, such as thermal gravimetric analysis (TGA), Fourier-transform infrared spectra (FTIR), ultraviolet-visible spectra (UV-Vis), X-ray diffraction (XRD), scanning electron microscopy (SEM) fitted with energy-dispersive X-ray analysis (EDX), and transmission electron microscopy (TEM), were used to characterize both NiO-olive and NiO-pure. The extract and nanoparticles were assessed for antiparasitic activity against adult ticks () and antimicrobial activity against .
From XRD, the crystal sizes of NiO-olive and NiO-pure were 32.94 nm and 13.85 nm, respectively. TGA, FTIR, and EDX showed the presence of olive organic residues in NiO-olive and their absence in NiO-pure. SEM and TEM showed an asymmetrical structure of NiO-olive and a regular, semi-spherical structure of NiO-pure. UV-Vis spectra showed surface plasmon resonance of NPs. Antiparasitic activity showed the highest mortality rate of 95% observed at a concentration of 0.06 mg/mL after four days of incubation. The antimicrobial activity showed the largest inhibition zone diameter of 33 ± 0.2 mm against the strain.
Nanoparticles of NiO-olive outperformed nanoparticles of NiO-pure and olive leaf extract in both antiparasitic and antimicrobial tests. These findings imply that NiO-olive may be widely used as an eco-friendly and effective antiparasitic and disinfection of sewage.
如今，在世界范围内，回收农业废弃物对于生产有价值的生物活性化合物和环境保护至关重要。橄榄叶生物活性化合物对制药工业有重大潜在影响。这些化合物具有显著的生物学特性，包括抗菌、抗病毒、抗炎、降血糖和抗氧化特性。
本研究展示了一种绿色合成方法，使用水相废弃的橄榄叶提取物制备氧化镍纳米粒子（NiO-olive）。将 NiO-olive 在 500°C 下煅烧得到纯氧化镍纳米粒子（NiO-pure）。采用热重分析（TGA）、傅里叶变换红外光谱（FTIR）、紫外-可见光谱（UV-Vis）、X 射线衍射（XRD）、扫描电子显微镜（SEM）结合能谱分析（EDX）和透射电子显微镜（TEM）等多种技术对 NiO-olive 和 NiO-pure 进行了表征。评估了提取物和纳米粒子对成年蜱（）的抗寄生虫活性和对 的抗菌活性。
从 XRD 可知，NiO-olive 和 NiO-pure 的晶体尺寸分别为 32.94nm 和 13.85nm。TGA、FTIR 和 EDX 表明 NiO-olive 中存在橄榄有机残留物，而 NiO-pure 中则不存在。SEM 和 TEM 显示 NiO-olive 具有不对称结构，NiO-pure 具有规则的半球形结构。紫外-可见光谱显示 NPs 存在表面等离子体共振。抗寄生虫活性显示，在孵育 4 天后，浓度为 0.06mg/mL 时观察到 95%的最高死亡率。抗菌活性显示对 菌株的最大抑菌圈直径为 33±0.2mm。
NiO-olive 纳米粒子在抗寄生虫和抗菌试验中的表现均优于 NiO-pure 纳米粒子和橄榄叶提取物。这些发现表明，NiO-olive 可能广泛用作环保且有效的抗寄生虫和污水消毒剂。
