Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, China.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1927-1936. doi: 10.1016/j.ijbiomac.2021.11.024. Epub 2021 Nov 5.
This study investigated the therapeutic effects and mechanisms of chitosans (CSs) with different molecular weights on ulcerative colitis (UC). Three size classes of CSs (Mw ≤ 3, 50, and 200 kDa) were used in this study. The effect of large CSs (Mw ≤ 200 kDa) on UC was the best, followed by that of medium CSs (Mw ≤ 50 kDa), and that of small CSs (Mw ≤ 3 kDa) was the least in the LPS-induced Raw 264.7 cell model and DSS-induced UC mice model. The therapeutic mechanisms of three CSs are related to anti-oxidation, anti-inflammation, and regulation of immunoglobulin and intestinal flora by attenuating body weight loss, decreasing the disease activity index (DAI) and MPO activity, suppressing proinflammatory cytokines and IgG levels, down-regulating the level of oxidative stress, increasing anti-inflammatory cytokines, SOD activity and Prevotellaceae_UCG-001 levels, and reducing the abundance of Proteobacteria, Actinobacteria, and Escherichia-Shigella. In general, the molecular weight of CSs influences their efficacy against UC. CSs with an optimal molecular weight demonstrate good development prospects for ameliorating UC.
本研究探讨了不同分子量壳聚糖（CSs）对溃疡性结肠炎（UC）的治疗作用和机制。本研究使用了三种分子量等级的 CSs（Mw≤3、50 和 200 kDa）。在 LPS 诱导的 Raw 264.7 细胞模型和 DSS 诱导的 UC 小鼠模型中，大分子量 CSs（Mw≤200 kDa）的效果最好，其次是中分子量 CSs（Mw≤50 kDa），小分子量 CSs（Mw≤3 kDa）的效果最差。三种 CSs 的治疗机制与抗氧化、抗炎以及通过减轻体重减轻、降低疾病活动指数（DAI）和 MPO 活性、抑制促炎细胞因子和 IgG 水平、下调氧化应激水平、增加抗炎细胞因子、SOD 活性和普雷沃氏菌科_UCG-001 水平、减少变形菌门、放线菌门和大肠杆菌-志贺氏菌的丰度有关。总的来说，CSs 的分子量影响其对 UC 的疗效。具有最佳分子量的 CSs 对改善 UC 具有良好的发展前景。
