Departments of Chemical and Physical Sciences, Cell and Systems Biology, Chemistry, and Physics, University of Toronto, Toronto, Canada.
Gut Microbes. 2021 Jan-Dec;13(1):1994832. doi: 10.1080/19490976.2021.1994832.
Nissle (EcN), a probiotic bacterium, has been employed in treating inflammatory bowel disease, but the nature of its therapeutic effect is not fully understood. Intestinal inflammation alters the environment, exposing the microbial population to new stresses and eliciting transcriptional responses. We administered EcN to germ-free mice and then compared its transcriptional response between DSS-treated and untreated conditions using RNA-seq analysis to identify 187 differentially expressed genes (119 upregulated, 68 downregulated) and verifying a subset with qRT-PCR. The upregulated genes included many involved in flagella biosynthesis and motility, as well as several members of the formate hydrogenlyase complex. Despite prior evidence that these pathways are both transcriptionally regulated by nitric oxide, tests did not establish that nitric oxide exposure alone elicited the transcriptional response. The results provide new information on the transcriptional response of EcN to inflammation and establish a basis for further investigation of its anti-inflammatory activity.
尼森（EcN）是一种益生菌细菌，已被用于治疗炎症性肠病，但它的治疗效果的性质尚不完全清楚。肠道炎症改变了环境，使微生物种群面临新的压力，并引发转录反应。我们将 EcN 给予无菌小鼠，然后使用 RNA-seq 分析比较 DSS 处理和未处理条件下的转录反应，以鉴定 187 个差异表达基因（119 个上调，68 个下调），并用 qRT-PCR 验证了其中一部分。上调的基因包括许多与鞭毛生物合成和运动有关的基因，以及甲酸氢酶复合物的几个成员。尽管先前有证据表明这些途径都受到一氧化氮的转录调控，但测试并未确定一氧化氮暴露本身是否会引发转录反应。研究结果为 EcN 对炎症的转录反应提供了新的信息，并为进一步研究其抗炎活性奠定了基础。
