Indicuen, a new hopane from Hale growing in Vietnam.

One new hopane-type triterpene, indicuen (), along with eight known compounds () were isolated from the -hexane extract of the lichen Hale. The chemical structures of isolated compounds were identified by interpretation of their spectroscopic data (1D, 2D NMR and HRESIMS) combined with DFT-NMR chemical shift calculations and subsequent assignment of DP4+ probabilities and by comparison with the literature. Indicuen represents for a rare hopane bearing a 1-carboxyethyl substituent at C-21 in lichens. Compounds and - were evaluated for -glucosidase inhibition and cytotoxicity against K562 and HepG2 cancer cell lines. Compounds , and exhibited moderate -glucosidase inhibition with IC values of 201.1, 156.3 and 187.4 µM, respectively. Compound also showed weak cytotoxicity toward K562 cell line while others showed no activity.