在使用微型螺钉治疗期间给予壳聚糖漱口水的正畸患者中白细胞介素-1α和白细胞介素-1β、环氧化酶-2和诱导型一氧化氮合酶mRNA表达的评估
Evaluation of IL-1α and IL-1β, COX-2, and iNOS mRNA Expression in Orthodontic Patients Given Chitosan Mouthwash During Treatment with Miniscrew.
作者信息
Anggani Haru Setyo, Hasriati Erlina, Winiati Bachtiar Endang
机构信息
Department of Orthodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
出版信息
J Int Soc Prev Community Dent. 2021 Sep 21;11(5):561-565. doi: 10.4103/jispcd.JISPCD_163_21. eCollection 2021 Sep-Oct.
OBJECTIVE
Chitosan is a biomaterial with antibacterial properties that may benefit from maintaining peri-miniscrew hygiene and preventing inflammation. This study aimed to evaluate the expression of inflammatory-related molecules from the gingival crevicular fluid (GCF) after treatment of 1% chitosan when compared with 0.2% chlorhexidine mouthwash of patients with orthodontic miniscrew.
MATERIALS AND METHODS
A total of 30 subjects were divided into three groups: the first group received mouthwash containing 1% chitosan, the second group 0.2% chlorhexidine digluconate, and the control group received aquadest. The GCF was collected before and after 4 days of rinsing, and relative expressions of IL-1α and IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) were evaluated by real-time qPCR.
RESULTS
The expression of IL-1α was the highest in chitosan-treated patients when compared with that of IL-1β in between-groups. Patients receiving chlorhexidine have the highest expression of COX-2 and iNOS when compared with the chitosan and control groups, respectively.
CONCLUSION
A mouthwash containing 1% of chitosan could suppress the expression of inflammatory mediators IL-1β, COX-2, and iNOS.
目的
壳聚糖是一种具有抗菌特性的生物材料，可能有助于保持微螺钉周围的卫生并预防炎症。本研究旨在评估正畸微螺钉患者使用1%壳聚糖治疗后，龈沟液（GCF）中炎症相关分子的表达，并与0.2%氯己定漱口水进行比较。
材料与方法
总共30名受试者被分为三组：第一组接受含1%壳聚糖的漱口水，第二组接受0.2%葡萄糖酸氯己定，对照组接受蒸馏水。在漱口4天前后收集龈沟液，并通过实时定量聚合酶链反应评估白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、环氧合酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的相对表达。
结果
与组间的IL-1β相比，壳聚糖治疗患者中IL-1α的表达最高。与壳聚糖组和对照组相比，接受氯己定治疗的患者中COX-2和iNOS的表达分别最高。
结论
含1%壳聚糖的漱口水可抑制炎症介质IL-1β、COX-2和iNOS的表达。
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