Mansjur Karima Qurnia, Erwansyah Eka, Pawinru Ardiansyah S, Nasir Mansjur, Djais Arni Irawaty, Sumule Virgino Calvine, Attaya Nurnabilla Syfadewi, Arinawaty Dian Yosi, Hutami Islamy Rahma, Permana Andi Dian
Department of Orthodontics, Faculty of Dentistry, Hasanuddin University, Indonesia.
Department of Periodontology, Faculty of Dentistry, Hasanuddin University, Indonesia.
J Oral Biol Craniofac Res. 2025 May-Jun;15(3):463-471. doi: 10.1016/j.jobcr.2025.02.014. Epub 2025 Mar 11.
Peri-implantitis, exacerbated microbial growth characterized by progressive bone loss and soft-tissue inflammation, significantly contributes to miniscrew failure during orthodontic treatment. Using a natural antibacterial coating presents an innovative approach to combat bacterial colonization. Sea urchin () spines containing chitosan (CS) exhibit notable antibacterial properties and biocompatibility effects. This study investigates the antimicrobial potential of CS from sea urchin spines applied onto the surfaces of orthodontic miniscrews, aiming to mitigate the impact of peri-implantitis.
The surface functional groups, phase composition, and crystal structure of CS were investigated using traditional examination methods alongside energy-dispersive X-ray analysis. The antibacterial activity of CS was evaluated against three bacteria by the disk diffusion method, minimum bacterial concentration (MBC), and minimum inhibitory concentration (MIC). Stainless steel miniscrews were coated with CS, and the surface was characterized by scanning electron microscopy (SEM).
Sea urchin-derived chitosan demonstrated significant antibacterial effects against key oral pathogens associated with peri-implantitis, with minimum inhibitory concentrations (MICs) of 16 ppm against and 32 ppm for both and . The minimum bactericidal concentrations (MBCs) were 4 ppm for and 16 ppm for both and indicating its strong bactericidal potential. Scanning electron microscopy (SEM) revealed that sea urchin chitosan effectively adhered to the surface of orthodontic miniscrews, showcasing its potential as a functional antimicrobial coating. These results emphasize the capability of sea urchin chitosan to target key oral pathogens, offering a promising approach to enhance microbial resistance and improve outcomes in orthodontic treatments.
种植体周围炎以进行性骨质流失和软组织炎症为特征，会加剧微生物生长，是正畸治疗中微型螺钉失败的重要原因。使用天然抗菌涂层是对抗细菌定植的一种创新方法。含有壳聚糖（CS）的海胆刺具有显著的抗菌性能和生物相容性效应。本研究调查了应用于正畸微型螺钉表面的海胆刺壳聚糖的抗菌潜力，旨在减轻种植体周围炎的影响。
采用传统检测方法结合能量色散X射线分析研究壳聚糖的表面官能团、相组成和晶体结构。通过纸片扩散法、最低杀菌浓度（MBC）和最低抑菌浓度（MIC）评估壳聚糖对三种细菌的抗菌活性。用壳聚糖对不锈钢微型螺钉进行涂层，并通过扫描电子显微镜（SEM）对其表面进行表征。
海胆来源的壳聚糖对与种植体周围炎相关的关键口腔病原体显示出显著的抗菌作用，对变形链球菌的最低抑菌浓度（MIC）为16 ppm，对牙龈卟啉单胞菌和具核梭杆菌均为32 ppm。对变形链球菌的最低杀菌浓度（MBC）为4 ppm，对牙龈卟啉单胞菌和具核梭杆菌均为16 ppm，表明其具有很强的杀菌潜力。扫描电子显微镜（SEM）显示，海胆壳聚糖能有效附着在正畸微型螺钉表面，展示了其作为功能性抗菌涂层的潜力。这些结果强调了海胆壳聚糖针对关键口腔病原体的能力，为增强微生物抗性和改善正畸治疗效果提供了一种有前景的方法。
