Tuyarum Chiraprapha, Songsang Aporn, Lertworapreecha Monthon
Microbiology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung, 93210, Thailand.
Faculty of Technology and Community Development, Thaksin University, Phatthalung, 93210, Thailand.
Vet World. 2021 May;14(5):1133-1142. doi: 10.14202/vetworld.2021.1133-1142. Epub 2021 May 11.
Using antimicrobials as a feed additive in swine production is prohibited because it is a major cause of the emergence of antimicrobial-resistant bacteria. Probiotics such as spp. are an attractive alternative to reduce antimicrobial resistance and promote swine growth. This study aimed to evaluate the probiotic properties of isolated from indigenous swine manure.
A total of 30 fecal samples from healthy individual indigenous pigs were collected and isolated on de Man, Rogosa, and Sharpe agar. The preliminary screen identified candidates with antibacterial activity against six pathogens and >50% survival and tolerance to acid (pH 3.0) and 1% bile salt. Isolates that passed the initial screen will be tested for other probiotic properties.
Of the 314 isolates from 30 pig manure samples, 17 isolates satisfied all initial conditions for probiotic properties. Each isolate has unique, distinctive properties. Isolates B4, B5, B8, B17, B87, and B144 formed thick biofilms, whereas isolates B5, B8, and 27 adhered well to the intestinal wall and exhibited strong autoaggregation properties. Isolate B4 aggregated with Enterohemorrhagic and Enteropathogenic . Tests in pH-adjusted cell-free medium indicated that the antibacterial activity resulted from bacterial acidification rather than bacteriocin formation. Sequence analysis () revealed 16 of the isolates were , and only one isolate was .
We isolated 17 from swine manure and demonstrated that their probiotic properties might be useful as a probiotic cocktail for swine feed.
在猪生产中使用抗菌剂作为饲料添加剂是被禁止的，因为它是抗菌耐药菌出现的主要原因。诸如 spp. 等益生菌是减少抗菌耐药性和促进猪生长的有吸引力的替代物。本研究旨在评估从本地猪粪便中分离出的 的益生菌特性。
从健康的个体本地猪收集了总共30份粪便样本，并在德氏、罗格斯和夏普琼脂上进行分离。初步筛选确定了对六种病原体具有抗菌活性且在酸（pH 3.0）和1%胆盐条件下存活率和耐受性>50%的候选菌株。通过初步筛选的分离株将测试其他益生菌特性。
从30份猪粪便样本中分离出的314株菌株中，有17株满足益生菌特性的所有初始条件。每个分离株都有独特、鲜明的特性。分离株B4、B5、B8、B17、B87和B144形成厚生物膜，而分离株B5、B8和27与肠壁粘附良好并表现出强自聚集特性。分离株B4与肠出血性 和肠致病性 聚集。在pH调节的无细胞培养基中的测试表明，抗菌活性是由细菌酸化而非细菌素形成导致的。序列分析（）显示16株分离株为 ，只有一株分离株为 。
我们从猪粪便中分离出17株 ，并证明它们的益生菌特性可能作为猪饲料的益生菌组合有用。
