Bernards R, Destree A, McKenzie S, Gordon E, Weinberg R A, Panicali D
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6854-8. doi: 10.1073/pnas.84.19.6854.
We have constructed a vaccinia virus recombinant that expresses the extracellular domain of the rat neu oncogene-encoded protein, a 185-kDa transmembrane glycoprotein termed p185. Strain NFS mice immunized with this recombinant virus developed a strong antibody response against the neu oncogene product and were fully protected against subsequent tumor challenge with neu-transformed NIH 3T3 cells. No tumor immunoprotection was found when recombinant virus-immunized mice were challenged with Ha-ras-transformed NIH 3T3 cells. These data indicate that immunization with a single oncogene-encoded antigen can fully and specifically protect animals against tumor cells bearing this antigen.
我们构建了一种痘苗病毒重组体，它表达大鼠neu癌基因编码蛋白的胞外结构域，这是一种称为p185的185 kDa跨膜糖蛋白。用这种重组病毒免疫的NFS品系小鼠针对neu癌基因产物产生了强烈的抗体反应，并对随后用neu转化的NIH 3T3细胞进行的肿瘤攻击具有完全的抵抗力。当用重组病毒免疫的小鼠受到Ha-ras转化的NIH 3T3细胞攻击时，未发现肿瘤免疫保护作用。这些数据表明，用单一癌基因编码抗原进行免疫可全面且特异性地保护动物免受携带该抗原的肿瘤细胞的侵害。
