Ding Yanmei, Yao Wangyuan, Fakhar-E-Alam Kulyar Muhammad, Mo Quan, Pan Huachun, Zhang Yan, Ma Bingjie, He Ya, Zhang Mengdi, Hong Jiajia, Waqas Muhammad, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
Ecotoxicol Environ Saf. 2021 Nov 12;228:112981. doi: 10.1016/j.ecoenv.2021.112981.
Thiram causes tibial dyschondroplasia in broilers, leading to a significant economic loss in the poultry industry. Our study explored the effects of taurine in thiram induced tibial dyschondroplasia (TD) through in vivo and in vitro approches. In in vivo study, thiram resulted in lameness disorder, low production parameters ALP, ACP, and a high level of NOS. While, the taurine exhibited promising effect by reducing lameness, increasing ALP, ACP levels, and significantly lowering NOS level with the restoration of the growth plate. In in vitro study, thiram caused distortion and disintegration of chondrocytes. The CCK-8 technique revealed the lower cell activity in TD as compared with the treatment group. Even, the treatment and taurine groups had higher cell activity than control group. Also, the chondrocyte morphology progressively reverted to normal after taurine treatment. It might effectively decreased the symptoms of TD in broilers and their production performance. Further research found that the taurine effectively improved chondrocytes' cell viability and recovered lameness disorder by regulation of HIF-1α, VEGFA, and Wnt/β-catenin signaling pathways. In summary, these results indicate that taurine has a protective effect on thiram-induced broilers and it can enhance the growth activity by directly affecting the development of chondrocytes and blood vessels.
福美双可导致肉鸡胫骨软骨发育不良，给家禽业造成重大经济损失。我们的研究通过体内和体外方法探讨了牛磺酸对福美双诱导的胫骨软骨发育不良（TD）的影响。在体内研究中，福美双导致跛行障碍、低生产参数碱性磷酸酶（ALP）、酸性磷酸酶（ACP）以及高水平的一氧化氮合酶（NOS）。而牛磺酸通过减轻跛行、提高ALP和ACP水平以及显著降低NOS水平并恢复生长板，表现出了良好的效果。在体外研究中，福美双导致软骨细胞变形和崩解。CCK - 8技术显示，与治疗组相比，TD组细胞活性较低。甚至，治疗组和牛磺酸组的细胞活性均高于对照组。此外，牛磺酸处理后软骨细胞形态逐渐恢复正常。它可能有效减轻肉鸡TD症状及其生产性能。进一步研究发现，牛磺酸通过调节缺氧诱导因子 - 1α（HIF - 1α）、血管内皮生长因子A（VEGFA）和Wnt/β - 连环蛋白信号通路，有效提高软骨细胞的细胞活力并恢复跛行障碍。总之，这些结果表明牛磺酸对福美双诱导的肉鸡具有保护作用，并且它可以通过直接影响软骨细胞和血管的发育来增强生长活性。
