Golshah Amin, Omidi Khaled, Nikkerdar Nafiseh, Moradpoor Hedaiat, Ghorbani Fatemeh
Department of Orthodontic, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Student Research Committee Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Dent. 2021 Nov 8;2021:8451522. doi: 10.1155/2021/8451522. eCollection 2021.
Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats.
Twenty-eight male Wistar rats were randomized into four groups ( = 7). The first molar and central incisor were connected using a nickel-titanium (NiTi) coil spring with a 50 g load in each rat. The two experimental groups received 0.75 mg/kg and 1.5 mg/kg MTX, respectively, intraperitoneally for 21 days. The negative control group did not receive any injection and did not undergo orthodontic treatment. The positive control group underwent orthodontic treatment and received 0.9% saline (NaCl) injections for 21 days. All rats were sacrificed with chloroform inhalation after 21 days; their maxilla was resected, and the mean number of Howship's lacunae, blood vessels, osteoclasts, and resorption lacunae was counted. The reduction in bone volume (bone volume to total volume ratio (BV/TV)) at the site of the maxillary molar was quantified by microcomputed tomography (micro-CT).
OTM, the number of osteoclasts, and the number of blood vessels significantly increased in rats treated with MTX ( < 0.05). However, the increase in the number of Howship's lacunae and resorption lacunae was not significant ( > 0.05). Lower BV/TV in the MTX groups was in agreement with the increased number of osteoclasts.
Injection of MTX can significantly increase OTM and decrease root resorption in rats.
对于正畸医生而言，了解药物、维生素及各种补充剂对正畸牙齿移动（OTM）的影响至关重要。本研究旨在评估甲氨蝶呤（MTX）注射对大鼠OTM的影响。
将28只雄性Wistar大鼠随机分为四组（每组 = 7只）。在每只大鼠中，使用镍钛（NiTi）螺旋弹簧连接第一磨牙和中切牙，并施加50 g的负载。两个实验组分别腹腔注射0.75 mg/kg和1.5 mg/kg的MTX，持续21天。阴性对照组未接受任何注射且未接受正畸治疗。阳性对照组接受正畸治疗并注射0.9%生理盐水（NaCl），持续21天。21天后，所有大鼠通过吸入氯仿处死；切除其上颌骨，计数豪氏陷窝、血管、破骨细胞和吸收陷窝的平均数量。通过微计算机断层扫描（micro-CT）定量上颌磨牙部位的骨体积减少（骨体积与总体积之比（BV/TV））。
MTX治疗的大鼠中，OTM、破骨细胞数量和血管数量显著增加（P < 0.05）。然而，豪氏陷窝和吸收陷窝数量的增加并不显著（P > 0.05）。MTX组较低的BV/TV与破骨细胞数量的增加一致。
注射MTX可显著增加大鼠的OTM并减少牙根吸收。
