Department of Joint Surgery, Shanghai East Hospital of Nanjing Medical University, Nanjing, 211166, China.
Department of Joint Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
J Mech Behav Biomed Mater. 2022 Jan;125:104972. doi: 10.1016/j.jmbbm.2021.104972. Epub 2021 Nov 11.
Poly(ether-ether-ketone) (PEEK) with good biocompatibility exhibits high mechanical strengths but bioinert. In addition, tantalum (Ta) possesses outstanding osteogenesis but high density and elastic modulus, and cost. In this study, by blending Ta nanoparticles with PEEK, Ta/PEEK composite (TP) was prepared, which was then treated by concentrated sulfuric acid to form a microporous surface containing Ta particles on TP (TPS). Moreover, genistein (GS) with antibacterial property was loaded into the microporous surface of TPS (TPSG). Compared with TP, the surface properties (e.g., surface roughness and hydrophilicity) of TPS was obviously improved because of the microporous surface including Ta nanoparticles. Moreover, TPS showed low antibacterial properties because of presence of sulfonic group while TPSG exhibited excellent antibacterial properties due to GS loaded into the microporous surface. Furthermore, compared with TP, TPS obviously promoted attachment and proliferation of MG63 cells, while TPSG with GS remarkably inducing osteogenic differentiation of the cells compared with TPS in vitro. Moreover, in comparison with TP, TPS with optimized surface properties promoted new bone regeneration and osseointegration, while TPSG loading GS further enhanced bone regeneration as well as osseointegration in vivo. In summary, the GS loaded into microporous surface including Ta nanoparticles of TPSG exhibited antibacterial and osteogenic activity, which would have great potential for bone tissue repair.
聚醚醚酮（PEEK）具有良好的生物相容性，表现出较高的机械强度，但生物惰性较大。此外，钽（Ta）具有出色的成骨能力，但密度和弹性模量较高，成本也较高。在本研究中，通过将 Ta 纳米粒子与 PEEK 共混，制备了 Ta/PEEK 复合材料（TP），然后用浓硫酸处理，在 TP 上形成含有 Ta 颗粒的微孔表面（TPS）。此外，具有抗菌性能的染料木黄酮（GS）被载入 TPS 的微孔表面（TPSG）中。与 TP 相比，TPS 的表面性质（如表面粗糙度和润湿性）因包含 Ta 纳米粒子的微孔表面而明显改善。此外，由于存在磺酸基团，TPS 表现出低抗菌性能，而 TPSG 由于负载到微孔表面的 GS 而表现出优异的抗菌性能。此外，与 TP 相比，TPS 明显促进了 MG63 细胞的黏附和增殖，而 TPSG 中的 GS 明显促进了细胞的成骨分化，优于 TPS 体外。此外，与 TP 相比，具有优化表面性能的 TPS 促进了新骨的再生和骨整合，而负载 GS 的 TPSG 进一步增强了体内的骨再生和骨整合。总之，负载到 TPSG 中 Ta 纳米粒子微孔表面的 GS 具有抗菌和成骨活性，在骨组织修复方面具有巨大的潜力。
