Kouhpayeh Hamid Reza, Tabasi Farhad, Dehvari Mohammad, Naderi Mohammad, Bahari Gholamreza, Khalili Tahereh, Clark Courtney, Ghavami Saeid, Taheri Mohsen
Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Transl Med Commun. 2021;6(1):26. doi: 10.1186/s41231-021-00106-0. Epub 2021 Nov 17.
The COVID-19 pandemic remains an emerging public health crisis with serious adverse effects. The disease is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV--2) infection, targeting angiotensin-converting enzyme-2 (ACE2) receptor for cell entry. However, changes in the renin-angiotensin system (RAS) balance alter an individual's susceptibility to COVID-19 infection. We aimed to evaluate the association between AGT rs699 C > T, ACE rs4646994 I/D, and AGTR1 rs5186 C > A variants and the risk of COVID-19 infection and the severity in a sample of the southeast Iranian population.
A total of 504 subjects, including 258 COVID-19 positives, and 246 healthy controls, were recruited. Genotyping of the ACE gene rs4646994, and AGT rs699, and AGTR1 rs5186 polymorphisms was performed by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP), respectively.
Our results showed that the II genotype of ACE rs4646994 and the I allele decreased the risk of COVID-19 infection. Moreover, we found that the TC genotype and C allele of AGT rs699 increased the risk of COVID-19 infection. The AGTR1 rs5186 was not associated with COVID-19 infection. Also, we did not find any association between these polymorphisms and the severity of the disease. However, we found a significantly higher age and prevalence of diabetes and hypertension in patients with severe disease than a non-severe disease.
These findings suggest that ACE rs4646994 and AGT rs699 polymorphisms increase the risk of COVID-19 infection in a southeast Iranian population.
新型冠状病毒肺炎（COVID-19）大流行仍然是一场新出现的公共卫生危机，具有严重的不良影响。该疾病由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染引起，其通过靶向血管紧张素转换酶2（ACE2）受体进入细胞。然而，肾素-血管紧张素系统（RAS）平衡的改变会改变个体对COVID-19感染的易感性。我们旨在评估伊朗东南部人群样本中AGT基因rs699 C>T、ACE基因rs4646994 I/D和AGTR1基因rs5186 C>A变异与COVID-19感染风险及严重程度之间的关联。
共招募了504名受试者，其中包括258名COVID-19阳性患者和246名健康对照。分别通过聚合酶链反应（PCR）和PCR-限制性片段长度多态性（PCR-RFLP）对ACE基因rs4646994、AGT基因rs699和AGTR1基因rs5186多态性进行基因分型。
我们的结果表明，ACE基因rs4646994的II基因型和I等位基因降低了COVID-19感染风险。此外，我们发现AGT基因rs699的TC基因型和C等位基因增加了COVID-19感染风险。AGTR1基因rs5186与COVID-19感染无关。而且，我们未发现这些多态性与疾病严重程度之间存在任何关联。然而，我们发现重症患者的年龄、糖尿病和高血压患病率显著高于非重症患者。
这些发现表明，ACE基因rs4646994和AGT基因rs699多态性增加了伊朗东南部人群感染COVID-19的风险。
