育龄期健康体重女性与超重/肥胖女性的阴道微生物组。
The vaginal microbiome in women of reproductive age with healthy weight versus overweight/obesity.
机构信息
Department of Pediatrics, Division of Endocrinology and Diabetes, Penn State Health, Hershey, Pennsylvania, USA.
Department of Pediatrics, Division of Endocrinology, Diabetes, and Metabolism, Virginia Commonwealth University, Richmond, Virginia, USA.
出版信息
Obesity (Silver Spring). 2022 Jan;30(1):142-152. doi: 10.1002/oby.23306. Epub 2021 Nov 21.
OBJECTIVE
The aim of this study was to evaluate the differences between the vaginal microbiome of reproductive-aged women with overweight and obesity (Ow/Ob) compared with healthy weight (HW).
METHODS
In this case-control study, a cohort of 367 nonpregnant women (18 to 40 years) with Ow/Ob (BMI ≥25 kg/m ) was case-matched with 367 women with HW (BMI 18.0 to 24.9 kg/m ). The study was a secondary analysis of 16S rRNA vaginal microbiome surveys through the Vaginal Human Microbiome Study (VaHMP). Groups were matched on age, race/ethnicity, income, and nulliparity status.
RESULTS
Mean age and BMI of Ow/Ob and HW groups were 26.8 versus 26.7 years and 37.0 versus 22.1 kg/m , respectively. The overall vaginal microbiome composition differed between groups (PERMANOVA, p = 0.035). Women with Ow/Ob had higher alpha diversity compared with women with HW (Wilcoxon test, Shannon index p = 0.025; inverse Simpson index p = 0.026). Lactobacillus dominance (≥30% proportional abundance) was observed in a greater proportion of women with HW (48.7%) compared with Ow/Ob (40.1%; p = 0.026).
CONCLUSIONS
The vaginal microbiome differs in reproductive-aged women with Ow/Ob compared with women with HW, with increased alpha diversity and decreased predominance of Lactobacillus. Observed differences in the vaginal microbiome may partially explain differences in preterm birth and bacterial vaginosis risk between these populations.
目的
本研究旨在评估超重和肥胖（Ow/Ob）与健康体重（HW）的育龄妇女阴道微生物组之间的差异。
方法
在这项病例对照研究中，对 367 名非孕妇（18 至 40 岁）的 Ow/Ob（BMI≥25kg/m²）进行队列研究，并与 367 名 HW（BMI 18.0 至 24.9kg/m²）进行病例匹配。该研究是通过阴道人类微生物组研究（VaHMP）对 16S rRNA 阴道微生物组调查进行的二次分析。组间匹配年龄、种族/民族、收入和初产状况。
结果
Ow/Ob 和 HW 组的平均年龄和 BMI 分别为 26.8 岁和 26.7 岁和 37.0 千克/m²和 22.1 千克/m²。两组之间的整体阴道微生物组组成存在差异（PERMANOVA，p=0.035）。Ow/Ob 组的 alpha 多样性高于 HW 组（Wilcoxon 检验，Shannon 指数 p=0.025；逆 Simpson 指数 p=0.026）。HW 组中 Lactobacillus 优势（≥30%的比例丰度）的比例高于 Ow/Ob 组（48.7%比 40.1%；p=0.026）。
结论
与 HW 相比，Ow/Ob 育龄妇女的阴道微生物组存在差异，alpha 多样性增加，Lactobacillus 优势减少。这些人群之间在阴道微生物组方面观察到的差异可能部分解释了早产和细菌性阴道病风险的差异。
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