Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Quartier Vallée 2, Avenue de Cureghem 3, 4000, Liège, Belgium.
Department of Food Sciences - Microbiology, Faculty of Veterinary Medicine, University of Liège, Quartier Vallée 2, Avenue de Cureghem 3, 4000, Liège, Belgium.
BMC Vet Res. 2021 Nov 24;17(1):361. doi: 10.1186/s12917-021-03055-w.
Extrinsic and intrinsic factors have been shown to influence nasal microbiota (NM) in humans. Very few studies investigated the association between nasal microbiota and factors such as facial/body conformation, age, and environment in dogs. The objectives are to investigate variations in NM in healthy dogs with different facial and body conformations. A total of 46 dogs of different age, living environment and from 3 different breed groups were recruited: 22 meso-/dolichocephalic medium to large breed dogs, 12 brachycephalic dogs and 12 terrier breeds. The nasal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V1-V3 regions) amplicons.
We showed major differences in the NM composition together with increased richness and α-diversity in brachycephalic dogs, compared to meso-/dolichocephalic medium to large dogs and dogs from terrier breeds.
Healthy brachycephalic breeds and their unique facial conformation is associated with a distinct NM profile. Description of the NM in healthy dogs serves as a foundation for future researches assessing the changes associated with disease and the modulation of NM communities as a potential treatment.
已证实，外在和内在因素会影响人类的鼻腔微生物群（NM）。只有很少的研究调查了鼻腔微生物群与狗的面部/身体形态、年龄和环境等因素之间的关系。本研究旨在调查不同面部和身体形态的健康犬的 NM 变化。共招募了 46 只不同年龄、生活环境和来自 3 个不同品种组的犬：22 只中/长头型中型至大型犬、12 只短头型犬和 12 只梗犬。通过 16S rRNA 基因（V1-V3 区）扩增子的测序评估鼻腔细菌微生物群。
与中/长头型中型至大型犬和梗犬相比，我们发现短头型犬的 NM 组成存在主要差异，同时丰富度和 α 多样性增加。
健康的短头型品种及其独特的面部形态与独特的 NM 特征相关。描述健康犬的 NM 为未来评估与疾病相关的变化以及作为潜在治疗方法的 NM 群落的调节提供了基础。
