Righetti Laura, Dall'Asta Chiara, Lucini Luigi, Battilani Paola
Department of Food and Drug, University of Parma, Parma, Italy.
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Piacenza, Italy.
Front Plant Sci. 2021 Nov 8;12:701680. doi: 10.3389/fpls.2021.701680. eCollection 2021.
Fumonisin-contaminated maize ( L.) products are a major health concern because of their toxic effects in humans and animals. Breeding maize for increased mycotoxin resistance is one of the key sustainable strategies for mitigating the effects of fumonisin contamination. Recent studies suggest a link between fumonisin accumulation and plant lipid and oxylipin profiles. However, the data collected so far do not reveal a cause-and-effect relationship. In this study, to decipher the multifactorial nature of mycotoxin resistance and plant-pathogen interaction mechanisms, we examined the oxylipin and complex lipid profiles of two maize hybrids (H21 and H22, the latter showing significantly lower FBs content) grown in the open field in two locations over 3years. Untargeted ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight (UHPLC-Q-TOF), together with chemometrics analysis, successfully distinguished between the two hybrids as having low- and high-level fumonisin contamination. Considering that H21 and H22 were exposed to the same environmental factors, the higher activation of lipid signaling systems in H22 suggests that other routes are enabled in the less susceptible hybrids to limit fumonisin B (FB) accumulation. Our results highlighted the crucial role played by oxylipin and sphingolipid signaling in modulating the complex maize response to infection. Overall, our results returned a global view on the changes in lipid metabolites related to fumonisin accumulation under open field conditions, and revealed a strong activation of the lipid signaling cascade in maize in the presence of FB.
受伏马菌素污染的玉米（L.）产品是一个主要的健康问题，因为它们对人和动物具有毒性作用。培育抗真菌毒素能力更强的玉米是减轻伏马菌素污染影响的关键可持续战略之一。最近的研究表明伏马菌素积累与植物脂质和氧化脂质谱之间存在联系。然而，迄今为止收集的数据并未揭示因果关系。在本研究中，为了解析抗真菌毒素能力和植物 - 病原体相互作用机制的多因素性质，我们检测了在两个地点的露天环境中种植3年的两个玉米杂交种（H21和H22，后者伏马菌素B1含量显著较低）的氧化脂质和复合脂质谱。非靶向超高效液相色谱与四极杆 - 飞行时间质谱联用（UHPLC - Q - TOF），并结合化学计量学分析，成功区分了伏马菌素污染水平低和高的两个杂交种。鉴于H21和H22暴露于相同的环境因素，H22中脂质信号系统的更高激活表明在较不易感的杂交种中存在其他途径来限制伏马菌素B（FB）的积累。我们的结果突出了氧化脂质和鞘脂信号在调节玉米对感染的复杂反应中所起的关键作用。总体而言，我们的结果给出了在露天条件下与伏马菌素积累相关的脂质代谢物变化的全局视图，并揭示了在存在FB的情况下玉米中脂质信号级联的强烈激活。
