De Giani Alessandra, Bovio Federica, Forcella Matilde Emma, Lasagni Marina, Fusi Paola, Di Gennaro Patrizia
Department of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milano, Italy.
Department of Earth and Environmental Sciences, University of Milano-Bicocca, 20126 Milano, Italy.
Foods. 2021 Oct 21;10(11):2536. doi: 10.3390/foods10112536.
Maitake () is a medicinal mushroom known for its peculiar biological activities due to the presence of functional components, including dietary fibers and glucans, that can improve human health through the modulation of the gut microbiota. In this paper, a Maitake ethanol/water extract was prepared and characterized through enzymatic and chemical assays. The prebiotic potential of the extract was evaluated by the growth of some probiotic strains and of a selected probiotic consortium. The results revealed the prebiotic properties due to the stimulation of the growth of the probiotic strains, also in consortium, leading to the production of SCFAs, including lactic, succinic, and valeric acid analyzed via GC-MSD. Then, their beneficials effect were employed in evaluating the vitality of three different healthy and tumoral colorectal cell lines (CCD841, CACO-2, and HT-29) and the viability rescue after co-exposure to different stressor agents and the probiotic consortium secondary metabolites. These metabolites exerted positive effects on colorectal cell lines, in particular in protection from reactive oxygen species.
舞茸（）是一种药用蘑菇，因其含有膳食纤维和葡聚糖等功能成分而具有独特的生物活性，这些成分可通过调节肠道微生物群来改善人类健康。本文制备了舞茸乙醇/水提取物，并通过酶法和化学分析法对其进行了表征。通过一些益生菌菌株和选定的益生菌联合体的生长来评估提取物的益生元潜力。结果表明，该提取物具有益生元特性，因为它能刺激益生菌菌株的生长，包括联合体中的菌株，从而导致产生短链脂肪酸，通过气相色谱-质谱联用仪（GC-MSD）分析发现其中包括乳酸、琥珀酸和戊酸。然后，利用它们的有益作用评估了三种不同的健康和肿瘤性结肠直肠细胞系（CCD841、CACO-2和HT-29）的活力，以及在共同暴露于不同应激源和益生菌联合体次生代谢产物后的活力恢复情况。这些代谢产物对结肠直肠细胞系产生了积极影响，尤其是在保护细胞免受活性氧的侵害方面。
