Liu Zhengjie, Lu Hongyun, Zhang Xinglin, Chen Qihe
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
J Fungi (Basel). 2021 Oct 20;7(11):887. doi: 10.3390/jof7110887.
is a famous and precious edible mushroom (Huang Mogu) on the Qinghai-Tibet plateau that has a unique flavor and remarkable medical functions. Herein, we report a reference-grade 27 Mb genome of containing 7068 protein-coding genes. The genome component and gene functions were predicted. Genome ontology enrichment and pathway analyses indicated the potential production capacity for terpenoids, polyketides and polysaccharides. Moreover, 16 putative gene clusters and 145 genes coding for secondary metabolites were obtained, including guadinomine and melleolides. In addition, phylogenetic and comparative genomic analyses shed light on the precise classification of suggesting that it belongs to the genus instead of . RNA-sequencing and comparative transcriptomic analysis revealed differentially expressed genes during four developmental stages of , that of which helps to identify important genes regulating fruiting body formation for strain modification. This study will provide insight into artificial cultivation and increase the production of useful metabolites.
是青藏高原上一种著名且珍贵的食用菌（黄蘑菇），具有独特风味和显著的药用功能。在此，我们报道了一个参考级别的27 Mb基因组，其包含7068个蛋白质编码基因。对基因组组成和基因功能进行了预测。基因本体富集和通路分析表明其具有萜类化合物、聚酮化合物和多糖的潜在生产能力。此外，获得了16个假定的基因簇和145个编码次生代谢产物的基因，包括胍丁胺和蜜环菌素。另外，系统发育和比较基因组分析为的精确分类提供了线索，表明它属于属而非属。RNA测序和比较转录组分析揭示了在四个发育阶段中差异表达的基因，这有助于识别调控子实体形成的重要基因以用于菌株改良。本研究将为人工栽培提供见解并增加有用代谢产物的产量。
