Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, P.R. China.
Key Laboratory of Crop Molecular Breeding of Qinghai Province, Xining, Qinghai, P.R. China.
J Basic Microbiol. 2018 Jun;58(6):554-563. doi: 10.1002/jobm.201700579. Epub 2018 Mar 23.
Floccularia luteovirens, an important edible mushroom widely distributed in the Qinghai-Tibet plateau, is ecologically important as an ectomycorrhizal fungus and can form the fairy ring. To explore the influence of F. luteovirens fairy ring on soil microbial communities, we compared the soil microbial communities in three different fairy ring zones (inside the fairy ring (IN); beneath the fairy ring (ON); and outside the fairy ring (OUT)). A total of 1.77 million bacterial reads and 1.59 million fungal reads were obtained. Moreover, sequence clustering yielded 519,613 (57,735 per sample) bacterial OTUs, and 513,204 (57,023 per sample) fungal OTUs representing. Microbial diversity was lower in samples from the ON zone compared with the other two zones. Mycorrhiza helper bacteria (MHB) such as Bradyrhizobium and Paenibacillus were more common in the ON zone, and we isolated four potential MHB from rhizosphere soil. Canonical correspondence analysis showed that the soil nutritional condition and physical changes caused by F. luteovirens shaped the microbial communities in the ON zone. This is the first report on the study of soil microbial diversity influenced by fairy ring F. luteovirens, and further studies need to be conducted to study the ecological function influenced by this species.
黄韧伞，一种广泛分布于青藏高原的重要食用蘑菇，作为外生菌根真菌具有重要的生态学意义，并且能够形成蕈环。为了探究黄韧伞蕈环对土壤微生物群落的影响，我们比较了三个不同蕈环区域（蕈环内（IN）；蕈环下（ON）；蕈环外（OUT））的土壤微生物群落。共获得了 177 万条细菌序列和 159 万条真菌序列。此外，序列聚类产生了 519,613 个（每个样本 57,735 个）细菌 OTUs 和 513,204 个（每个样本 57,023 个）真菌 OTUs。与其他两个区域相比，ON 区域的样本微生物多样性较低。ON 区域中常见的外生菌根菌辅助细菌（MHB）如慢生根瘤菌和巨大芽孢杆菌，我们从根际土壤中分离出了四种潜在的 MHB。典范对应分析表明，黄韧伞引起的土壤营养状况和物理变化塑造了 ON 区域的微生物群落。这是首次报道黄韧伞蕈环对土壤微生物多样性的影响，需要进一步研究来研究该物种影响的生态功能。
