Xing Rui, Gao Qing-Bo, Zhang Fa-Qi, Fu Peng-Cheng, Wang Jiu-Li, Yan Hui-Ying, Chen Shi-Long
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining Qinghai, 810001, P. R. China.
Key Laboratory of Crop Molecular Breeding of Qinghai Provice, Qinghai, P. R. China.
J Microbiol. 2017 Aug;55(8):600-606. doi: 10.1007/s12275-017-7101-4. Epub 2017 Jul 4.
Floccularia luteovirens, as an ectomycorrhizal fungus, is widely distributed in the Qinghai-Tibet Plateau. As an edible fungus, it is famous for its unique flavor. Former studies mainly focus on the chemical composition and genetic structure of this species. However, the phylogenetic relationship between genotypes remains unknown. In this study, the genetic variation and phylogenetic relationship between the genotypes of F. luteovirens in Qinghai-Tibet Plateau was estimated through the analysis on two protein-coding genes (rpb1 and ef-1α) from 398 individuals collected from 24 wild populations. The sample covered the entire range of this species during all the growth seasons from 2011 to 2015. 13 genotypes were detected and moderate genetic diversity was revealed. Based on the results of network analysis, the maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BI) analyses, the genotypes H-1, H-4, H-6, H-8, H-10, and H-11 were grouped into one clade. Additionally, a relatively higher genotype diversity (average h value is 0.722) and unique genotypes in the northeast edge of Qinghai- Tibet plateau have been found, combined with the results of mismatch analysis and neutrality tests indicated that Southeast Qinghai-Tibet plateau was a refuge for F. luteovirens during the historical geological or climatic events (uplifting of the Qinghai-Tibet Plateau or Last Glacial Maximum). Furthermore, the present distribution of the species on the Qinghai-Tibet plateau has resulted from the recent population expansion. Our findings provide a foundation for the future study of the evolutionary history and the speciation of this species.
黄绿蜜环菌作为一种外生菌根真菌，广泛分布于青藏高原。作为一种食用菌，它以其独特的风味而闻名。以往的研究主要集中在该物种的化学成分和遗传结构上。然而，基因型之间的系统发育关系仍然未知。在本研究中，通过对从24个野生种群收集的398个个体的两个蛋白质编码基因（rpb1和ef-1α）进行分析，估计了青藏高原黄绿蜜环菌基因型之间的遗传变异和系统发育关系。样本涵盖了该物种在2011年至2015年所有生长季节的整个分布范围。检测到13个基因型，并揭示了中等程度的遗传多样性。基于网络分析、最大似然法（ML）、最大简约法（MP）和贝叶斯推断（BI）分析的结果，基因型H-1、H-4、H-6、H-8、H-10和H-11被归为一个分支。此外，在青藏高原东北边缘发现了相对较高的基因型多样性（平均h值为0.722）和独特的基因型，结合错配分析和中性检验的结果表明，青藏高原东南部在历史地质或气候事件（青藏高原隆升或末次盛冰期）期间是黄绿蜜环菌的避难所。此外，该物种目前在青藏高原的分布是近期种群扩张的结果。我们的研究结果为该物种的进化历史和物种形成的未来研究提供了基础。
