Rydz Leszek, Wróbel Maria, Jurkowska Halina
Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 7 Kopernika St., 31-034 Kraków, Poland.
Antioxidants (Basel). 2021 Oct 29;10(11):1738. doi: 10.3390/antiox10111738.
Mitochondria are the key organelles of Fe-S cluster synthesis. They contain the enzyme cysteine desulfurase, a scaffold protein, iron and electron donors, and specific chaperons all required for the formation of Fe-S clusters. The newly formed cluster can be utilized by mitochondrial Fe-S protein synthesis or undergo further transformation. Mitochondrial Fe-S cluster biogenesis components are required in the cytosolic iron-sulfur cluster assembly machinery for cytosolic and nuclear cluster supplies. Clusters that are the key components of Fe-S proteins are vulnerable and prone to degradation whenever exposed to oxidative stress. However, once degraded, the Fe-S cluster can be resynthesized or repaired. It has been proposed that sulfurtransferases, rhodanese, and 3-mercaptopyruvate sulfurtransferase, responsible for sulfur transfer from donor to nucleophilic acceptor, are involved in the Fe-S cluster formation, maturation, or reconstitution. In the present paper, we attempt to sum up our knowledge on the involvement of sulfurtransferases not only in sulfur administration but also in the Fe-S cluster formation in mammals and yeasts, and on reconstitution-damaged cluster or restoration of enzyme's attenuated activity.
线粒体是铁硫簇合成的关键细胞器。它们含有半胱氨酸脱硫酶、一种支架蛋白、铁和电子供体以及铁硫簇形成所需的特定伴侣蛋白。新形成的簇可用于线粒体铁硫蛋白合成或进行进一步转化。线粒体铁硫簇生物合成成分是胞质铁硫簇组装机制中胞质和核簇供应所必需的。作为铁硫蛋白关键成分的簇很脆弱，暴露于氧化应激时容易降解。然而，一旦降解，铁硫簇可以重新合成或修复。有人提出，负责将硫从供体转移到亲核受体的硫转移酶、硫氰酸酶和3-巯基丙酮酸硫转移酶参与了铁硫簇的形成、成熟或重构。在本文中，我们试图总结我们关于硫转移酶不仅在硫管理方面，而且在哺乳动物和酵母中铁硫簇形成以及重构受损簇或恢复酶减弱活性方面的知识。
