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2015 - 2019年波兰能力验证结果。

Results of Proficiency Testing for in Poland, 2015-2019.

作者信息

Różycki Mirosław, Korpysa-Dzirba Weronika, Bełcik Aneta, Bilska-Zając Ewa, Karamon Jacek, Sroka Jacek, Zdybel Jolanta, Cencek Tomasz

机构信息

Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Partyzantow Avenue 57, 24-100 Pulawy, Poland.

出版信息

J Clin Med. 2021 Nov 18;10(22):5389. doi: 10.3390/jcm10225389.

Abstract

Trichinellosis is a zoonotic meat-borne disease caused by the nematodes of the genus . Meat containing live larvae is a source of infection. The examination of meat for was introduced in 1869, but the digestion method for this did not appear in Poland until the late 1970s. Nowadays, the meat of all food animals susceptible to spp. is examined in the frame of official control with the digestion method. The majority of laboratories in Poland meet the requirements of the ISO/IEC 17025 Standard (352 field laboratories). Laboratory personnel participate in quality control programs. This paper presents the results of proficiency tests (PTs) organized within 2015-2019 in Poland. Over this period, the laboratories examined 7580 samples (contamination levels: zero, one, three, and five larvae). Each laboratory was provided with a set of samples (one negative and three positive). Over 95% of the samples were considered correct in qualitative assessments, though the results of the quantitative evaluations were slightly lower, with 89% of samples being considered correct. Based on a sample evaluation, 88% of laboratories passed the PT comparison. A slight decrease was observed in the examination of samples spiked with five larvae, and great progress was achieved in samples containing three larvae. Low levels of sample contamination are sought after in laboratories but may make evaluations difficult. For this reason, we must consider increasing the number of larvae added to the samples in the next PTs.

摘要

旋毛虫病是一种由旋毛虫属线虫引起的人畜共患食源性疾病。含有活幼虫的肉类是感染源。1869年开始对肉类进行旋毛虫检查,但直到20世纪70年代末,波兰才出现针对旋毛虫的消化检查方法。如今,在官方检查框架内,采用消化方法对所有易感染旋毛虫属的食用动物的肉类进行检查。波兰的大多数实验室(352个现场实验室)符合ISO/IEC 17025标准的要求。实验室人员参与质量控制计划。本文介绍了2015年至2019年在波兰组织的能力验证测试(PT)的结果。在此期间,各实验室检查了7580个样本(污染水平:零、一、三、五只幼虫)。每个实验室都收到一组样本(一个阴性样本和三个阳性样本)。在定性评估中,超过95%的样本被认为是正确的,不过定量评估的结果略低,89%的样本被认为是正确的。根据样本评估,88%的实验室通过了PT比较。在检查添加五只幼虫的样本时观察到略有下降,而在检查含有三只幼虫的样本时取得了很大进展。实验室追求低水平的样本污染,但这可能会使评估变得困难。因此,我们必须考虑在下次PT中增加添加到样本中的幼虫数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74e3/8623675/d6d97fad5d76/jcm-10-05389-g001.jpg

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