用于体内检测金纳米颗粒标记免疫细胞的X射线荧光成像：基于GEANT4的可行性研究

X-ray-Fluorescence Imaging for In Vivo Detection of Gold-Nanoparticle-Labeled Immune Cells: A GEANT4 Based Feasibility Study.

作者信息

Ungerer Arthur, Staufer Theresa, Schmutzler Oliver, Körnig Christian, Rothkamm Kai, Grüner Florian

机构信息

University Medical Center Hamburg-Eppendorf, Department of Radiotherapy and Radiation Oncology, Medical Faculty, University of Hamburg, Martinistraße 52, 20246 Hamburg, Germany.

Universität Hamburg and Center for Free-Electron Laser Science (CFEL), Institute for Experimental Physics, Faculty of Mathematics, Informatics and Natural Sciences, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.

出版信息

Cancers (Basel). 2021 Nov 17;13(22):5759. doi: 10.3390/cancers13225759.

The growing field of cellular therapies in regenerative medicine and oncology calls for more refined diagnostic tools that are able to investigate and monitor the function and success of said therapies. X-ray Fluorescence Imaging (XFI) can be applied for molecular imaging with nanoparticles, such as gold nanoparticles (GNPs), which can be used in immune cell tracking. We present a Monte Carlo simulation study on the sensitivity of detection and associated radiation dose estimations in an idealized setup of XFI in human-sized objects. Our findings demonstrate the practicability of XFI in human-sized objects, as immune cell tracking with a minimum detection limit of 4.4 × 10 cells or 0.86 μg gold in a cubic volume of 1.78 mm can be achieved. Therefore, our results show that the current technological developments form a good basis for high sensitivity XFI.

再生医学和肿瘤学领域中不断发展的细胞疗法需要更精密的诊断工具，以便能够研究和监测上述疗法的功能及成效。X射线荧光成像（XFI）可用于纳米颗粒的分子成像，如金纳米颗粒（GNP），其可用于免疫细胞追踪。我们针对人体大小物体的XFI理想化设置中的检测灵敏度及相关辐射剂量估计进行了蒙特卡洛模拟研究。我们的研究结果证明了XFI在人体大小物体中的实用性，因为在1.78立方毫米的体积中，可实现对免疫细胞的追踪，最低检测限为4.4×10个细胞或0.86微克金。因此，我们的结果表明，当前的技术发展为高灵敏度XFI奠定了良好基础。