Demirci Sahin, Sahiner Mehtap, Suner Selin Sagbas, Sahiner Nurettin
Department of Chemistry, Faculty of Sciences and Arts, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale 17100, Turkey.
Nanoscience and Technology Research and Application Center, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale 17100, Turkey.
Micromachines (Basel). 2021 Oct 20;12(11):1280. doi: 10.3390/mi12111280.
Carbon nanotubes (CNTs) due to their outstanding mechanical, thermal, chemical, and optical properties were utilized as a base material and were coated with polydopamine (PDA) (PDA@CNT) via the simple self-polymerization of dopamine (DA). Then, PDA@CNT coatings of up to five layers were examined for potential biomedical applications. The success of multiple coating of CNTs with PDA was confirmed via increased weight loss values with the increased number of PDA coatings of CNTs at 500 °C by thermogravimetric analysis. The surface area of bare CNTs was measured as 263.9 m/g and decreased to 197.0 m/g after a 5th coating with PDA. Furthermore, the antioxidant activities of CNT and PDA@CNTs were determined via total flavonoid content (TFC), total phenol content (TPC), and Fe(III)-reducing antioxidant power (FRAP) tests, revealing the increased antioxidant ability of PDA@CNTs with the increasing numbers of PDA coatings. Moreover, a higher inhibition percentage of the activity of the alpha-glucosidase enzyme with 95.1 ± 2.9% inhibition at 6 mg/mL PDA-1st@CNTs concentration was found. The CNT and PDA@CNTs exhibited blood compatibility, less than a 2.5% hemolysis ratio, and more than 85% blood clotting indexes. The minimum inhibition concentration (MIC) of PDA-5th@CNTs against and bacteria was determined as 10 mg/mL.
碳纳米管（CNTs）因其出色的机械、热、化学和光学性能而被用作基础材料，并通过多巴胺（DA）的简单自聚合作用涂覆聚多巴胺（PDA）（PDA@CNT）。然后，对多达五层的PDA@CNT涂层进行了潜在生物医学应用的研究。通过热重分析在500℃下随着CNT上PDA涂层数量的增加失重值增加，证实了用PDA对CNT进行多次涂层的成功。未涂覆的CNT的表面积测量为263.9 m²/g，在第五次涂覆PDA后降至197.0 m²/g。此外，通过总黄酮含量（TFC）、总酚含量（TPC）和铁（III）还原抗氧化能力（FRAP）测试测定了CNT和PDA@CNT的抗氧化活性，结果表明随着PDA涂层数量的增加，PDA@CNT的抗氧化能力增强。此外，发现在6 mg/mL PDA-1st@CNT浓度下，α-葡萄糖苷酶活性的抑制率更高，为95.1±2.9%。CNT和PDA@CNT表现出血液相容性，溶血率小于2.5%，凝血指数大于85%。PDA-5th@CNT对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度（MIC）测定为10 mg/mL。
