Budziszewska Marta, Wieczorek Przemysław
Department of Molecular Biology and Biotechnology, Institute of Plant Protection-National Research Institute, Wegorka 20 Street, 60-318 Poznan, Poland.
Plants (Basel). 2021 Nov 13;10(11):2454. doi: 10.3390/plants10112454.
Tomato torrado virus (ToTV) induces severe systemic necrosis in . This work aimed at describing the genetic variability of necrosis-inducing ToTV-Wal'17 collected in 2017, derived from the ToTV-Wal'03 after long-term passages in plants. Sequence analyses of the ToTV-Wal'17 indicated twenty-eight single nucleotide substitutions in coding sequence of both RNAs, twelve of which resulted in amino acid changes in viral polyproteins. Moreover the sequencing data revealed that the 3'UTR of ToTV-Wal'17 RNA1 was 394 nts shorter in comparison to Wal'03. The performed sequence analyses revealed that 3'UTR of RNA1 of ToTV-Wal'17 is the most divergent across all previously described European isolates.
番茄烤焦病毒（ToTV）在……中引发严重的系统性坏死。这项工作旨在描述2017年收集的诱导坏死的ToTV-Wal'17的遗传变异性，它是ToTV-Wal'03在植物中经过长期传代后产生的。对ToTV-Wal'17的序列分析表明，两个RNA的编码序列中有28个单核苷酸替换，其中12个导致病毒多聚蛋白的氨基酸发生变化。此外，测序数据显示，与Wal'03相比，ToTV-Wal'17 RNA1的3'UTR短394个核苷酸。所进行的序列分析表明，ToTV-Wal'17 RNA1的3'UTR在所有先前描述的欧洲分离株中差异最大。
