在南非出现并全基因组分析。

Emergence and Full Genome Analysis of in South Africa.

机构信息

Discipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg 3209, South Africa.

Rural Agronomy and Development, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg 3209, South Africa.

出版信息

Viruses. 2020 Oct 15;12(10):1167. doi: 10.3390/v12101167.

DOI:10.3390/v12101167

PMID:33076368

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7602605/

Abstract

Emerging pests and diseases are a major threat to food production worldwide. In a recent survey, (ToTV) was identified on tomato crops in the Limpopo province of South Africa and a first report of the disease was published. In this follow-up study, the full genome sequence of a tomato-infecting isolate of ToTV from South Africa was elucidated. High-throughput sequencing was used to generate the full genome of ToTV infecting tomato crops in South Africa. The longest contig obtained for the RNA-1 and RNA-2 genome of ToTV was comprised of 7420 and 5381 nucleotides (nt), respectively. Blast analysis of the RNA-1 sequence of ToTV from South Africa (ToT-186) matched 99% to a Spanish and Polish isolate; the RNA-2 segment of ToTV from South Africa (ToT-186) matched 99% to ToTV isolates from Italy and Poland, respectively. The information presented in this study will go a long way towards better understanding the emergence and spread of ToTV and devising sustainable management of ToTV diseases.

摘要

新出现的病虫害是全球粮食生产的主要威胁。在最近的一项调查中，南非林波波省的番茄作物上发现了 ToTV（ToTV），并发表了该病的首次报告。在这项后续研究中，阐明了南非番茄感染 ToTV 的分离株的全基因组序列。高通量测序用于生成南非感染番茄作物的 ToTV 全基因组。ToTV 的 RNA-1 和 RNA-2 基因组最长的连续序列分别由 7420 和 5381 个核苷酸（nt）组成。对南非 ToTV（ToT-186）的 RNA-1 序列进行的 Blast 分析与西班牙和波兰的分离株匹配 99%；南非 ToTV 的 RNA-2 片段（ToT-186）与意大利和波兰的 ToTV 分离株分别匹配 99%。本研究提供的信息将有助于更好地了解 ToTV 的出现和传播，并制定 ToTV 病的可持续管理策略。